Application
Fig1: Sample: MCF-7(Human) Cell Lysate at 40 ug A431(Human) Cell Lysate at 40 ug U2os(Human) Cell Lysate at 40 ug Primary: Anti-ZNF185 (ER1918-95) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 76/39 kD Observed band size: 67 kDApplication
Fig2: Blank control: A431. Primary Antibody (green line): Rabbit Anti-Phospho-c-Fos (Ser32) antibody (bs-6199R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Product Name | ZNF185 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LIM domain protein ZNF185 P1 A Zinc finger protein 185 (LIM domain) ZN185_HUMAN. |
| Product description | May be involved in the regulation of cellular proliferation and/or differentiation. |
| Immunogen | KLH conjugated synthetic peptide derived from human ZNF185 551-650/689 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:3ug/Test: |
|
| Species Reactivity | HumanMouseRatDog |
| Concentration | 1mg/ml |
| Alternative Names | LIM domain protein ZNF185 P1 A Zinc finger protein 185 (LIM domain) ZN185_HUMAN. |
|---|---|
| Molecular Weight(MW) | 76(human)/39(mo, KDa |
| Cellular Localization | Cytoplasm, cytoskeleton. Cell junction, focal adhesion. |
| SwissProt ID | O15231 |
|---|
Application
Fig1: Sample: MCF-7(Human) Cell Lysate at 40 ug A431(Human) Cell Lysate at 40 ug U2os(Human) Cell Lysate at 40 ug Primary: Anti-ZNF185 (ER1918-95) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 76/39 kD Observed band size: 67 kD
Application
Fig2: Blank control: A431. Primary Antibody (green line): Rabbit Anti-Phospho-c-Fos (Ser32) antibody (bs-6199R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Positive Control | Expressed in placenta, pancreas and kidney. Also expressed in prostate, testis, ovary and blood. |
|---|---|
| Application Notes | WB:1:500-2000: FC:3ug/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
