Application
Fig1: Sample: Lane1: Testis (Mouse) Lysate at 30 ug Lane2: Small intestine (Mouse) Lysate at 30 ug Primary: Anti-ZNF300(ER1918-98) at 1:200 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1:3000 dilution; Predicted band size : 66kD Observed band size : 70,66kDApplication
Fig2: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-ZNF300 (ER1918-98) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kD Observed band size: 68 kD| Product Name | ZNF300 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Kruppel like zinc finger protein Zinc finger protein 300 ZN300_HUMAN. |
| Product description | Has a transcriptional repressor activity. |
| Immunogen | KLH conjugated synthetic peptide derived from human ZNF300 165-250/604 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | Kruppel like zinc finger protein Zinc finger protein 300 ZN300_HUMAN. |
|---|---|
| Molecular Weight(MW) | 68 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q96RE9 |
|---|
Application
Fig1: Sample: Lane1: Testis (Mouse) Lysate at 30 ug Lane2: Small intestine (Mouse) Lysate at 30 ug Primary: Anti-ZNF300(ER1918-98) at 1:200 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1:3000 dilution; Predicted band size : 66kD Observed band size : 70,66kD
Application
Fig2: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-ZNF300 (ER1918-98) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kD Observed band size: 68 kD| Positive Control | Expressed mostly in heart, skeletal muscle and brain. Isoform 1 and isoform 2 are highly expressed in testis. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
