Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (α-TAT) Polyclonal Antibody, Unconjugated (ER1919-19) at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | α-TAT |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C6orf134 Acetyltransferase mec 17 homolog Alpha TAT Alpha tubulin acetyltransferase 1 Alpha tubulin N acetyltransferase Chromosome 6 open reading frame 134 Hypothetical protein LOC79969 MEC17 Nbla00487 TAT ATAT_HUMAN. |
| Product description | Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. May affect microtubule stability and regulate microtubule dynamics. May be involved in neuron development. |
| Immunogen | KLH conjugated synthetic peptide derived from human alpha-TAT 1-100/421 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | C6orf134 Acetyltransferase mec 17 homolog Alpha TAT Alpha tubulin acetyltransferase 1 Alpha tubulin N acetyltransferase Chromosome 6 open reading frame 134 Hypothetical protein LOC79969 MEC17 Nbla00487 TAT ATAT_HUMAN. |
|---|---|
| Molecular Weight(MW) | 47 KDa |
| SwissProt ID | Q9H8X5 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (α-TAT) Polyclonal Antibody, Unconjugated (ER1919-19) at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
