Application
Fig1: Western blot analysis of Caspase-3 on different lysates using anti- Caspase-3 antibody at 1/1,000 dilution.Positive control:Lane 1: Human lung Lane 2: Human brainLane 3: Jurkat Lane 4: F9Lane 5: NCCTI Lane 6: DaudiLane 7: Raji Lane 8: MCF-7Lane 9: HelaApplication
Fig2: ICC staining of Caspase-3 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining of Caspase-3 in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Caspase-3 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Caspase-3 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Hela cells with Caspase-3 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.| Product Name | Caspase-3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | A830040C14Rik antibody Apopain antibody CASP-3 antibody CASP3 antibody CASP3_HUMAN antibody Casp3a antibody Caspase 3 antibody Caspase 3, apoptosis-related cysteine peptidase antibody Caspase 3, apoptosis-related cysteine protease antibody Caspase 3, apoptosis-related cysteine protease a antibody Caspase-3 subunit p12 antibody CC3 antibody CPP-32 antibody CPP32 antibody CPP32B antibody Cysteine protease CPP32 antibody EC 3.4.22.56 antibody LICE antibody mldy antibody OTTHUMP00000165052 antibody OTTHUMP00000165053 antibody OTTHUMP00000165054 antibody PARP cleavage protease antibody Procaspase3 antibody Protein Yama antibody SCA 1 antibody SCA-1 antibody SREBP cleavage activity 1 antibody Yama antibody |
| Product description | Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP). Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. |
| Immunogen | Synthetic peptide (KLH-coupled) witihin human Caspase-3 N-terminal. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:1,000 ICC:1:200 IHC:1:200 FC:1:100-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | A830040C14Rik antibody
Apopain antibody
CASP-3 antibody
CASP3 antibody
CASP3_HUMAN antibody
Casp3a antibody
Caspase 3 antibody
Caspase 3 apoptosis-related cysteine peptidase antibody Caspase 3 apoptosis-related cysteine protease antibody Caspase 3 apoptosis-related cysteine protease a antibody Caspase-3 subunit p12 antibody CC3 antibody CPP-32 antibody CPP32 antibody CPP32B antibody Cysteine protease CPP32 antibody EC 3.4.22.56 antibody LICE antibody mldy antibody OTTHUMP00000165052 antibody OTTHUMP00000165053 antibody OTTHUMP00000165054 antibody PARP cleavage protease antibody Procaspase3 antibody Protein Yama antibody SCA 1 antibody SCA-1 antibody SREBP cleavage activity 1 antibody Yama antibody |
|---|---|
| Molecular Weight(MW) | 31 kDa |
| Cellular Localization | Nucleus, cytoplasm, |
| SwissProt ID | P42574 |
|---|
Application
Fig1: Western blot analysis of Caspase-3 on different lysates using anti- Caspase-3 antibody at 1/1,000 dilution.Positive control:Lane 1: Human lung Lane 2: Human brainLane 3: Jurkat Lane 4: F9Lane 5: NCCTI Lane 6: DaudiLane 7: Raji Lane 8: MCF-7Lane 9: Hela
Application
Fig2: ICC staining of Caspase-3 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining of Caspase-3 in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Caspase-3 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Caspase-3 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Hela cells with Caspase-3 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.| Positive Control | Jurkat, F9, NCCIT, Hela, Raji, MCF-7, Daudi, A549, human tosil tissue, human liver cancer tissue, human lung tissue,human brain tissue,HepG2 |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:200 IHC:1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
