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Fig1: Western blot analysis of TGF-Beta 1 on different cell lysates using anti- TGF-Beta 1 antibody at 1/1000 dilution. Positive control:
Lane 1: Raji
Lane 2: MCF-2
Lane 3: A549
Lane 4: Human kidney
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Fig2: ICC staining TGF-Beta 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Fig3: ICC staining TGF-Beta 1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Fig4: ICC staining TGF-Beta 1 in SKBR-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TGF-Beta 1 antibody. Counter stained with hematoxylin.
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TGF-Beta 1 antibody. Counter stained with hematoxylin.
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Fig7: Flow cytometric analysis of HepG2 cells with TGF-Beta 1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.