Application
Fig1: Western blot analysis of Stat-1α/β on different cell lysates using anti-Stat-1α/β antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3 Lane 3: MCF-7Application
Fig2: ICC staining Stat-1α/β in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Stat-1α/β in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Stat-1α/β in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Stat-1α/β antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse skeletal human kidney tissue using anti-Stat-1α/β antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Stat-1α/β antibody. Counter stained with hematoxylin.Application
Fig8: Flow cytometric analysis of Hela cells with Stat-1α/β antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.| Product Name | Stat-1 alpha/beta |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CANDF7 antibody DKFZp686B04100 antibody ISGF 3 antibody ISGF3 antibody OTTHUMP00000163552 antibody OTTHUMP00000165046 antibody OTTHUMP00000165047 antibody OTTHUMP00000205845 antibody Signal transducer and activator of transcription 1 antibody Signal transducer and activator of transcription 1, 91kDa antibody Signal transducer and activator of transcription 1-alpha/beta antibody Stat1 antibody STAT1_HUMAN antibody STAT91 antibody Transcription factor ISGF-3 components p91/p84 antibody |
| Product description | STAT1 is a member of the Signal Transducers and Activators of Transcription family of transcription factors. STAT1 is involved in upregulating genes due to a signal by either type I, type II, or type III interferons. In response to IFN-γ stimulation, STAT1 forms homodimers or heterodimers with STAT3 that bind to the GAS (Interferon-Gamma-Activated Sequence) promoter element; in response to either IFN-α or IFN-β stimulation, STAT1 forms a heterodimer with STAT2 that can bind the ISRE (Interferon-Stimulated Response Element) promoter element. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:1,000 ICC:1:200 IHC:1:200, FC:1:100-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | CANDF7 antibody
DKFZp686B04100 antibody
ISGF 3 antibody
ISGF3 antibody
OTTHUMP00000163552 antibody
OTTHUMP00000165046 antibody
OTTHUMP00000165047 antibody
OTTHUMP00000205845 antibody
Signal transducer and activator of transcription 1 antibody
Signal transducer and activator of transcription 1 91kDa antibody Signal transducer and activator of transcription 1-alpha/beta antibody Stat1 antibody STAT1_HUMAN antibody STAT91 antibody Transcription factor ISGF-3 components p91/p84 antibody |
|---|---|
| Molecular Weight(MW) | 87 kDa |
| Cellular Localization | Cytoplasm, nucleus. |
| SwissProt ID | P42224 |
|---|
Application
Fig1: Western blot analysis of Stat-1α/β on different cell lysates using anti-Stat-1α/β antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3 Lane 3: MCF-7
Application
Fig2: ICC staining Stat-1α/β in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Stat-1α/β in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Stat-1α/β in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Stat-1α/β antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse skeletal human kidney tissue using anti-Stat-1α/β antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Stat-1α/β antibody. Counter stained with hematoxylin.
Application
Fig8: Flow cytometric analysis of Hela cells with Stat-1α/β antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.| Positive Control | MCF-7, Hela, NIH/3T3, HepG2, human kidney tissue, human breast cancer tissue,mouse kidney tissue. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:200 IHC:1:200, FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
