Application
Fig1: Western blot analysis of TOP2A on different cell lysates using anti-TOP2A antibody at 1/500 dilution. Positive control: Lane 1: Jurkat Lane 2: NIH/3T3 Lane 3: A431 Lane 4: L929 Lane 5: A549 Lane 6: Human liver Lane 7: HUVECApplication
Fig2: ICC staining TOP2A in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining TOP2A in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining TOP2A in A431 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: ICC staining TOP2A in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TOP2A antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TOP2A antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TOP2A antibody. Counter stained with hematoxylin.Application
Fig9: Flow cytometric analysis of Hela cells with TOP2A antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.| Product Name | Topoisomerase II alpha |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | alpha isozyme antibody ATP hydrolyzing DNA topoisomerase II alfa antibody DNA gyrase antibody DNA topoisomerase (ATP hydrolyzing) antibody DNA topoisomerase 2 alpha antibody DNA topoisomerase 2-alpha antibody DNA topoisomerase II 170 kD antibody DNA topoisomerase II alpha isozyme antibody DNA topoisomerase II antibody DNA Topoisomerase2 antibody TOP 2A antibody TOP2 antibody TOP2A antibody TOP2A_HUMAN antibody Topoisomerase DNA II alpha 170kDa antibody TP2A antibody |
| Product description | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. It is essential during mitosis and meiosis for proper segregation of daughter chromosomes. |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500 ICC:1:200 IHC:1:200 FC:1:100-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | alpha isozyme antibody ATP hydrolyzing DNA topoisomerase II alfa antibody DNA gyrase antibody DNA topoisomerase (ATP hydrolyzing) antibody DNA topoisomerase 2 alpha antibody DNA topoisomerase 2-alpha antibody DNA topoisomerase II 170 kD antibody DNA topoisomerase II alpha isozyme antibody DNA topoisomerase II antibody DNA Topoisomerase2 antibody TOP 2A antibody TOP2 antibody TOP2A antibody TOP2A_HUMAN antibody Topoisomerase DNA II alpha 170kDa antibody TP2A antibody |
|---|---|
| Molecular Weight(MW) | 174 kDa |
| Cellular Localization | Cytoplasm, nucleus. |
| SwissProt ID | P11388 |
|---|
Application
Fig1: Western blot analysis of TOP2A on different cell lysates using anti-TOP2A antibody at 1/500 dilution. Positive control: Lane 1: Jurkat Lane 2: NIH/3T3 Lane 3: A431 Lane 4: L929 Lane 5: A549 Lane 6: Human liver Lane 7: HUVEC
Application
Fig2: ICC staining TOP2A in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining TOP2A in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining TOP2A in A431 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: ICC staining TOP2A in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TOP2A antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TOP2A antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TOP2A antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of Hela cells with TOP2A antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.| Positive Control | A549, Hela, NIH/3T3, Jurkat, L929, A431, HUVEC, human liver tissue, human tonsil tissue, human breast cancer tissue, human colon cancer tissue. |
|---|---|
| Application Notes | WB:1:500 ICC:1:200 IHC:1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
