Application
Fig1: Western blot analysis of MMP2 on PC12 cell lysates using anti-MMP2 antibody at 1/500 dilution.Application
Fig2: ICC staining MMP2 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining MMP2 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-MMP2 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-MMP2 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-MMP2 antibody. Counter stained with hematoxylin.| Product Name | MMP2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 72 kDa gelatinase antibody 72kD type IV collagenase antibody CLG 4 antibody CLG 4A antibody CLG4 antibody CLG4A antibody Collagenase Type 4 alpha antibody Collagenase type IV A antibody Gelatinase A antibody Gelatinase alpha antibody Gelatinase neutrophil antibody Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase antibody Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) antibody Matrix Metalloproteinase 2 antibody Matrix metalloproteinase II antibody Matrix metalloproteinase-2 antibody MMP 2 antibody MMP II antibody MMP-2 antibody MMP2 antibody MMP2_HUMAN antibody MONA antibody Neutrophil gelatinase antibody PEX antibody TBE 1 antibody TBE-1 antibody |
| Product description | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Activation of MMP-2 requires proteolytic processing. A complex of membrane type 1 MMP (MT1-MMP/MMP14) and tissue inhibitor of metalloproteinase 2 recruits pro-MMP 2 from the extracellular milieu to the cell surface. Activation then requires an active molecule of MT1-MMP and auto catalytic cleavage. Clustering of integrin chains promotes activation of MMP-2. Mutations in the MMP2 gene are associated with Torg-Winchester syndrome, multicentric osteolysis, arthritis syndrome, and possibly keloids. |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:1,000 ICC:1:200 IHC:1:200 FC:1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 72 kDa gelatinase antibody
72kD type IV collagenase antibody
CLG 4 antibody
CLG 4A antibody
CLG4 antibody
CLG4A antibody
Collagenase Type 4 alpha antibody
Collagenase type IV A antibody
Gelatinase A antibody
Gelatinase alpha antibody
Gelatinase neutrophil antibody
Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase antibody
Matrix metalloproteinase 2 (gelatinase A 72kDa gelatinase 72kDa type IV collagenase) antibody Matrix Metalloproteinase 2 antibody Matrix metalloproteinase II antibody Matrix metalloproteinase-2 antibody MMP 2 antibody MMP II antibody MMP-2 antibody MMP2 antibody MMP2_HUMAN antibody MONA antibody Neutrophil gelatinase antibody PEX antibody TBE 1 antibody TBE-1 antibody |
|---|---|
| Molecular Weight(MW) | 74 kDa |
| Cellular Localization | Cytoplasm, secreted |
| SwissProt ID | P08253 |
|---|
Application
Fig1: Western blot analysis of MMP2 on PC12 cell lysates using anti-MMP2 antibody at 1/500 dilution.
Application
Fig2: ICC staining MMP2 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining MMP2 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-MMP2 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-MMP2 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-MMP2 antibody. Counter stained with hematoxylin.| Positive Control | PC12, HepG2, Ags, Hela, human lung cancer tissue, human colon cancer tissue, human breast tissue, human liver tissue. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:200 IHC:1:200 FC:1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
