Application
Fig1: ICC staining MuERVL-Gag in mouse 3-cell stage embryo (red). The cells were incubated overnight at +4°C with MuERVL-Gag rabbit pAb at a 1/100 dilution (shown in red) and phalloidin (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).| Product Name | MuERVL-Gag |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | A large number of retrotransposons are expressed when the zygotic genome is first transcribed, including the endogenous retroviruses (ERVs), LINE-1 elements, and the non-autonomous SINE elements. At the 2C stage, MuERV-L/MERVL retrovirus-like elements are transiently de-repressed and produce 3% of the transcribed mRNAs. Following the 2C stage, MERVL-retroelement expression is silenced. The newly study discovered that this regulated pattern of MERVL expression overlapped with greater than one hundred 2C-specific genes that have co-opted regulatory elements from these foreign retroviruses to initiate their transcription. |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC |
| ICC:1:200 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Molecular Weight(MW) | 67 kDa |
|---|
| SwissProt ID | V9H130 |
|---|
Application
Fig1: ICC staining MuERVL-Gag in mouse 3-cell stage embryo (red). The cells were incubated overnight at +4°C with MuERVL-Gag rabbit pAb at a 1/100 dilution (shown in red) and phalloidin (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).| Positive Control | Mouse embryo. |
|---|---|
| Application Notes | ICC:1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
