WB
Western Blot analysis of various cells using EphA1 Polyclonal Antibody.WB
Western blot analysis of lysates from COLO205 cells, using EPHA1 Antibody. The lane on the right is blocked with the synthesized peptide.IHC
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).ICC/IF
Immunofluorescence analysis of HeLa cells, using EPHA1 Antibody. The picture on the right is blocked with the synthesized peptide.| Product Name | EPHA1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human EPHA1. AA range:541-590 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:300 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | EPHA1 antibody EPH antibody EPHT antibody EPHT1 antibody Ephrin type-A receptor 1 antibody hEpha1 antibody EPH tyrosine kinase antibody EPH tyrosine kinase 1 antibody Erythropoietin-producing hepatoma receptor antibody Tyrosine-protein kinase receptor EPH antibody |
|---|---|
| Molecular Weight(MW) | 108 kDa |
| Cellular Localization | Cell membrane ; Single-pass type I membrane protein. |
| SwissProt ID | P21709 |
|---|
WB
Western Blot analysis of various cells using EphA1 Polyclonal Antibody.
WB
Western blot analysis of lysates from COLO205 cells, using EPHA1 Antibody. The lane on the right is blocked with the synthesized peptide.
IHC
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
ICC/IF
Immunofluorescence analysis of HeLa cells, using EPHA1 Antibody. The picture on the right is blocked with the synthesized peptide.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:300 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
