WB
Western Blot analysis of 22RV1 cells using Phospho-CaMKIIβ/γ/δ (T287) Polyclonal Antibody diluted at 1:500.WB
Western blot analysis of lysates from rat brain, using CaMK2-beta/gamma/delta (Phospho-Thr287) Antibody. The lane on the right is blocked with the phospho peptide.IHC
Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,CaMKIIβ/γ/δ (phospho Thr287) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.IHC
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,CaMKIIβ/γ/δ (phospho Thr287) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.IHC
Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,CaMKIIβ/γ/δ (phospho Thr287) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.IHC
Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1,CaMKIIβ/γ/δ (phospho Thr287) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.ICC/IF
Immunofluorescence analysis of rat-spleen tissue. 1,CaMKIIβ/γ/δ (phospho Thr287) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.| Product Name | CaMKIIβ/γ/δ (Phospho Thr287) Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Alternative splicing results in multiple transcript variants. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CaMK2-beta/gamma/delta around the phosphorylation site of Thr287. AA range:253-302. |
| Modification | P-Thr287 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:100-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | CAMK2B antibody CAM2 antibody CAMK2 antibody CAMKB antibody Calcium/calmodulin-dependent protein kinase type II subunit beta antibody CaM kinase II subunit beta antibody CaMK-II subunit beta antibody CAMK2G antibody CAMK antibody CAMK-II antibody CAMKG antibody Calcium/calmodulin-dependent protein kinase type II subunit gamma antibody antibody |
|---|---|
| Molecular Weight(MW) | 50/65 kDa |
| Cellular Localization | Cytoplasm, cytoskeleton . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Sarcoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, synapse . In slow-twitch muscle, evenly distributed between longitudinal SR and junctional SR. |
WB
Western Blot analysis of 22RV1 cells using Phospho-CaMKIIβ/γ/δ (T287) Polyclonal Antibody diluted at 1:500.
WB
Western blot analysis of lysates from rat brain, using CaMK2-beta/gamma/delta (Phospho-Thr287) Antibody. The lane on the right is blocked with the phospho peptide.
IHC
Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,CaMKIIβ/γ/δ (phospho Thr287) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
IHC
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,CaMKIIβ/γ/δ (phospho Thr287) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
IHC
Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,CaMKIIβ/γ/δ (phospho Thr287) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
IHC
Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1,CaMKIIβ/γ/δ (phospho Thr287) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
ICC/IF
Immunofluorescence analysis of rat-spleen tissue. 1,CaMKIIβ/γ/δ (phospho Thr287) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
