| Product Name | CD3EAP |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD3EAP antibody; ASE1 antibody; CAST antibody; PAF49 antibody; DNA-directed RNA polymerase I subunit RPA34 antibody; A34.5 antibody; Antisense to ERCC-1 protein antibody; ASE-1 antibody; CD3-epsilon-associated protein antibody; CAST antibody; CD3E-associated protein antibody; RNA polymerase I-associated factor PAF49 antibody; |
| Product description | caution:It is not known whether the so-called human ASE1 and human CAST proteins represent two sides of a single gene product with sharply different functional characteristics. Experiments done with the mouse homolog protein are in favor of an implication of this gene in rRNA transcription instead of T-cell receptor signaling. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation. Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex. miscellaneous:It is in an antisense orientation to and overlaps the gene of the DNA repair enzyme ERCC1. This gene overlap is conserved in mouse suggesting an important biologic function. PTM:Isoform 1 is phosphorylated on tyrosine residues in initiation-competent Pol I-beta complexes but not in Pol I-alpha complexes. PTM:Isoform 2 undergoes tyrosine phosphorylation upon T-cell receptor (TCR) stimulation. This phosphorylation has not been confirmed by other group. PTM:Phosphorylated upon DNA damage, probably by ATM or ATR. Belongs to the eukaryotic RPA34 RNA polymerase subunit family. subcellular location:Found at the fibrillar centers of the nucleolus in interphase and during cell division it is localized to the nucleolus organizer regions of the chromosomes. subunit:Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits. Interacts with TAF1A thereby associates with the SL1 complex. Interacts with UBTF. Interacts with POLR1E/PRAF1 through its N-terminal region (By similarity). Isoform 2 interacts with CD3E. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CD3EAP. AA range:441-490 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500-1:2000 IHC:1:100-1:300 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CD3EAP antibody ASE1 antibody CAST antibody PAF49 antibody DNA-directed RNA polymerase I subunit RPA34 antibody A34.5 antibody Antisense to ERCC-1 protein antibody ASE-1 antibody CD3-epsilon-associated protein antibody CAST antibody CD3E-associated protein antibody RNA polymerase I-associated factor PAF49 antibody |
|---|---|
| Molecular Weight(MW) | 55 kDa |
| SwissProt ID | O15446 |
|---|
| Positive Control | Eye,Serum,Skin,Testis, |
|---|---|
| Application Notes | WB:1:500-1:2000 IHC:1:100-1:300 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
