WB
Western Blot analysis of SH-SY5Y cells using COL4A2 Polyclonal Antibody diluted at 1:2000.WB
Western Blot analysis of SH-SY5Y 293T NIH-3T3 COLO cells using COL4A2 Polyclonal Antibody diluted at 1:2000.IHC
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using Collagen IV alpha2 Antibody. The picture on the right is blocked with the synthesized peptide.ICC/IF
Immunofluorescence analysis of HeLa cells, using Collagen IV alpha2 Antibody. The picture on the right is blocked with the synthesized peptide.| Product Name | COL4A2 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. The C-terminal portion of the protein, known as canstatin, is an inhibitor of angiogenesis and tumor growth. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Collagen IV alpha2. AA range:151-200. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:300 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | COL4A2 |
|---|---|
| Gene Synonyms | ICH BSVD2 POREN2 |
| Gene Full Name | collagen type IV alpha 2 chain |
| Gene Summary | This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. The C-terminal portion of the protein, known as canstatin, is an inhibitor of angiogenesis and tumor growth. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 150 kDa |
| Cellular Localization | Secreted, extracellular space, extracellular matrix, basement membrane. |
| SwissProt ID | P08572 |
|---|
WB
Western Blot analysis of SH-SY5Y cells using COL4A2 Polyclonal Antibody diluted at 1:2000.
WB
Western Blot analysis of SH-SY5Y 293T NIH-3T3 COLO cells using COL4A2 Polyclonal Antibody diluted at 1:2000.
IHC
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using Collagen IV alpha2 Antibody. The picture on the right is blocked with the synthesized peptide.
ICC/IF
Immunofluorescence analysis of HeLa cells, using Collagen IV alpha2 Antibody. The picture on the right is blocked with the synthesized peptide.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:300 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
