| Product Name | EID-1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EID1 antibody; C15orf3 antibody; CRI1 antibody; RBP21 antibody; PNAS-22 antibody; PTD014 antibody; EP300-interacting inhibitor of differentiation 1 antibody; 21 kDa pRb-associated protein antibody; CREBBP/EP300 inhibitory protein 1 antibody; E1A-like inhibitor of differentiation 1 antibody; EID-1 antibody; |
| Product description | developmental stage:Expression decreased with development in ventricular tissue while remaining highly expressed in adult atrial tissue. In primary cultures of human skeletal myocytes, expression decreased during myogenic differentiation (at protein level). Interacts with RB1 and EP300 and acts as a repressor of MYOD1 transactivation. Inhibits EP300 and CBP histone acetyltransferase activity. May be involved in coupling cell cycle exit to the transcriptional activation of genes required for cellular differentiation. May act as a candidate coinhibitory factor for NR0B2 that can be directly linked to transcription inhibitory mechanisms. induction:Down-regulated in differentiating U937 leukemia cells. miscellaneous:Inhibition of MYOD1 may be partly due to the ability of EID1 to bind and inhibit EP300 histone acetyltransferase activity. PTM:Ubiquitinated in U-2OS osteosarcoma cells and is rapidly degraded by proteasome as cells exit the cell cycle exit. subcellular location:May shuttle between nucleus and cytoplasm. subunit:Interacts via its LXCXE motif with the entire pocket region of RB1. Interacts with EP300, NR0B2 and TRIM27. tissue specificity:Widely expressed. Most abundantly expressed in heart, skeletal muscle, pancreas, brain and testis. Expressed at much lower levels in placenta and peripheral blood leukocyte. Barely detectable in lung. Also weakly expressed in lung carcinoma A549 and various leukemia cell lines. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human EID1. AA range:71-120 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:500-1:2000 IHC:1:100-1:300 IF:1:100-1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | EID1 antibody C15orf3 antibody CRI1 antibody RBP21 antibody PNAS-22 antibody PTD014 antibody EP300-interacting inhibitor of differentiation 1 antibody 21 kDa pRb-associated protein antibody CREBBP/EP300 inhibitory protein 1 antibody E1A-like inhibitor of differentiation 1 antibody EID-1 antibody |
|---|---|
| Molecular Weight(MW) | 21 kDa |
| SwissProt ID | Q9Y6B2 |
|---|
| Positive Control | Brain,Neonatal brain,Pituitary tumor,Promyelocytic leukemia |
|---|---|
| Application Notes | WB:1:500-1:2000 IHC:1:100-1:300 IF:1:100-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
