| Product Name | eIF3ε |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EIF3F antibody; EIF3S5 antibody; Eukaryotic translation initiation factor 3 subunit F antibody; eIF3f antibody; Deubiquitinating enzyme eIF3f antibody; Eukaryotic translation initiation factor 3 subunit 5 antibody; eIF-3-epsilon antibody; eIF3 p47 antibody; |
| Product description | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. mass spectrometry: PubMed:17322308,mass spectrometry: PubMed:18599441,PTM:Phosphorylated. Phosphorylation is enhanced upon serum stimulation. Belongs to the eIF-3 subunit F family. Contains 1 MPN (JAB/Mov34) domain. subunit:Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of FRAP1 and RAPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human EIF3F. AA range:81-130 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500-1:2000 IHC:1:100-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | EIF3F antibody EIF3S5 antibody Eukaryotic translation initiation factor 3 subunit F antibody eIF3f antibody Deubiquitinating enzyme eIF3f antibody Eukaryotic translation initiation factor 3 subunit 5 antibody eIF-3-epsilon antibody eIF3 p47 antibody |
|---|---|
| Molecular Weight(MW) | 38 kDa |
| SwissProt ID | O00303 |
|---|
| Positive Control | Amygdala,Brain,Liver,Lung, |
|---|---|
| Application Notes | WB:1:500-1:2000 IHC:1:100-1:300 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
