| Product Name | Ksr-1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KSR1 antibody; KSR antibody; Kinase suppressor of Ras 1 antibody; |
| Product description | caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data. Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity. PTM:Phosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 protein kinase domain. subcellular location:In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery. subunit:Interacts with HSPCA/HSP90, YWHAB/14-3-3, CDC37, MAP2K/MEK, MARK3, PPP2R1A and PPP2CA. Also interacts with RAF and MAPK/ERK, in a Ras-dependent manner (By similarity). The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human KSR. AA range:358-407 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:500-1:2000 IHC:1:100-1:300 IF:1:100-1:500 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | KSR1 antibody KSR antibody Kinase suppressor of Ras 1 antibody |
|---|---|
| Molecular Weight(MW) | 115 kDa |
| SwissProt ID | Q8IVT5 |
|---|
| Positive Control | Brain,Epithelium,Platelet,Synovial membrane, |
|---|---|
| Application Notes | WB:1:500-1:2000 IHC:1:100-1:300 IF:1:100-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
