| Product Name | MYPT1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PPP1R12A antibody; MBS antibody; MYPT1 antibody; Protein phosphatase 1 regulatory subunit 12A antibody; Myosin phosphatase-targeting subunit 1 antibody; Myosin phosphatase target subunit 1 antibody; Protein phosphatase myosin-binding subunit antibody; |
| Product description | Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP. RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosph |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MYPT1. AA range:661-710 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500-1:2000 IHC:1:100-1:300 |
|
| Species Reactivity | HumanMouseRatMonkey |
| Concentration | 1mg/ml |
| Alternative Names | PPP1R12A antibody MBS antibody MYPT1 antibody Protein phosphatase 1 regulatory subunit 12A antibody Myosin phosphatase-targeting subunit 1 antibody Myosin phosphatase target subunit 1 antibody Protein phosphatase myosin-binding subunit antibody |
|---|---|
| Molecular Weight(MW) | 140 kDa |
| SwissProt ID | O14974 |
|---|
| Positive Control | Blood,Brain,Colon,Epithelium,Liver,Placenta,Platelet,T-cell |
|---|---|
| Application Notes | WB:1:500-1:2000 IHC:1:100-1:300 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
