Application
Fig1: Western blot analysis of Nox3 on A543 lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER64216, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.| Product Name | Nox3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NOX3 antibody; MOX2 antibody; NADPH oxidase 3 antibody; Mitogenic oxidase 2 antibody; MOX-2 antibody; gp91phox homolog 3 antibody; GP91-3 antibody; |
| Product description | This gene encodes a member of the NOX family of NADPH oxidases. These enzymes have the capacity to generate superoxide and other reactive oxygen species (ROS) and transport electrons across the plasma membrane. The ROS generated by family members have been implicated in numerous biological functions including host defense, posttranlational processing of proteins, cellular signaling, regulation of gene expression, and cell differentiation. The protein encoded by this gene is expressed predominantly in the inner ear and is involved in the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity., |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NOX3. AA range:287-336 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500-1:2000 IHC:1:100-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | NOX3 antibody MOX2 antibody NADPH oxidase 3 antibody Mitogenic oxidase 2 antibody MOX-2 antibody gp91phox homolog 3 antibody GP91-3 antibody |
|---|---|
| Molecular Weight(MW) | 65 kDa |
| Cellular Localization | Membrane |
| SwissProt ID | Q9HBY0 |
|---|
Application
Fig1: Western blot analysis of Nox3 on A543 lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER64216, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.| Positive Control | Fetal kidney, A549. |
|---|---|
| Application Notes | WB:1:500-1:2000 IHC:1:100-1:300 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
