| Product Name | p14 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDKN2A antibody; CDKN2 antibody; MLM antibody; Cyclin-dependent kinase inhibitor 2A antibody; isoform 4 antibody; p14ARF antibody; p19ARF antibody; |
| Product description | CDKN2A generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by CDKN2A, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. CDKN2A is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human p14 ARF. AA range:71-120 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:500-1:2000 IHC:1:100-1:300 IF:1:100-1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CDKN2A antibody CDKN2 antibody MLM antibody Cyclin-dependent kinase inhibitor 2A antibody isoform 4 antibody p14ARF antibody p19ARF antibody |
|---|---|
| Molecular Weight(MW) | 18 kDa |
| SwissProt ID | Q8N726 |
|---|
| Application Notes | WB:1:500-1:2000 IHC:1:100-1:300 IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
