| Product Name | Separase |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ESPL1 antibody; ESP1 antibody; KIAA0165 antibody; Separin antibody; Caspase-like protein ESPL1 antibody; Extra spindle poles-like 1 protein antibody; Separase antibody; |
| Product description | Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al. 2009 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human SEPARASE. AA range:767-816 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:500-1:2000 IHC:1:100-1:300 IF:1:100-1:500 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | ESPL1 antibody ESP1 antibody KIAA0165 antibody Separin antibody Caspase-like protein ESPL1 antibody Extra spindle poles-like 1 protein antibody Separase antibody |
|---|---|
| Molecular Weight(MW) | 230 kDa |
| SwissProt ID | Q14674 |
|---|
| Positive Control | Bone marrow,Epithelium, |
|---|---|
| Application Notes | WB:1:500-1:2000 IHC:1:100-1:300 IF:1:100-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
