WB
Western Blot analysis of jurkat cell lysate using TNF-α Polyclonal Antibody. Secondary antibodywas diluted at 1:20000.IHC
Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).IF-P
Immunofluorescence analysis of human-stomach tissue. 1,TNF-α Polyclonal Antibody(red) was diluted at 1:200(4℃,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B| Product Name | TNF-α |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TNFA. AA range:141-190 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:300 IF-P:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | TNF antibody TNFA antibody TNFSF2 antibody Tumor necrosis factor antibody Cachectin antibody TNF-alpha antibody Tumor necrosis factor ligand superfamily member 2 antibody TNF-a antibody |
|---|---|
| Molecular Weight(MW) | 16 kDa |
| SwissProt ID | P01375 |
|---|
WB
Western Blot analysis of jurkat cell lysate using TNF-α Polyclonal Antibody. Secondary antibodywas diluted at 1:20000.
IHC
Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
IF-P
Immunofluorescence analysis of human-stomach tissue. 1,TNF-α Polyclonal Antibody(red) was diluted at 1:200(4℃,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B| Positive Control | Blood,Peripheral blood leukocyte,Placenta,Prostatic carcinoma, |
|---|---|
| Application Notes | WB:1:500-1:2000 IHC:1:100-1:300 IF-P:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
