Application
Fig1: Western blot analysis of HNF-1β on Jurkat and HunEc cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER65431, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.| Product Name | HNF-1β |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HNF1B antibody; TCF2 antibody; Hepatocyte nuclear factor 1-beta antibody; HNF-1-beta antibody; HNF-1B antibody; Homeoprotein LFB3 antibody; Transcription factor 2 antibody; TCF-2 antibody; Variant hepatic nuclear factor 1 antibody; vHNF1 antibody; |
| Product description | This gene encodes a member of the homeodomain-containing superfamily of transcription factors. The protein binds to DNA as either a homodimer, or a heterodimer with the related protein hepatocyte nuclear factor 1-alpha. The gene has been shown to function in nephron development, and regulates development of the embryonic pancreas. Mutations in this gene result in renal cysts and diabetes syndrome and noninsulin-dependent diabetes mellitus, and expression of this gene is altered in some types of cancer. Multiple transcript variants encoding different isoforms have been found for this gene. As the name suggests, hepatocyte nuclear factors are expressed predominately in the liver. However HNFs are also expressed and play important roles in a number of other tissues so that the name hepatocyte nuclear factor is somewhat misleading. Nevertheless, the liver is the only tissue in which a significant number of different HNFs are expressed at the same time. In addition, there are a number of genes which contain multiple promoter and enhancer regions each regulated by a different HNF. Furthermore, efficient expression of these genes require synergistic activation by multiple HNFs. Hence hepatocyte nuclear factors function to ensure liver specific expression of certain genes. |
| Immunogen | Synthesized peptide derived from the N-terminal region of human HNF-1β. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | HNF1B antibody TCF2 antibody Hepatocyte nuclear factor 1-beta antibody HNF-1-beta antibody HNF-1B antibody Homeoprotein LFB3 antibody Transcription factor 2 antibody TCF-2 antibody Variant hepatic nuclear factor 1 antibody vHNF1 antibody |
|---|---|
| Molecular Weight(MW) | 60 kDa |
| Cellular Localization | Nucleus |
| SwissProt ID | P35680 |
|---|
Application
Fig1: Western blot analysis of HNF-1β on Jurkat and HunEc cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER65431, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.| Positive Control | Colon, Liver, Thalamus. |
|---|---|
| Application Notes | WB:1:500-1:2000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
