WB
Western blot analysis of FUNDC1 on different lysates with Rabbit anti-FUNDC1 antibody at 1/1,000 dilution. Lane 1: 293T cell lysate (20 µg/Lane), Lane 2: Jurkat cell lysate (20 µg/Lane), Lane 3: HepG2 cell lysate (20 µg/Lane), Lane 4: HeLa cell lysate (20 µg/Lane), Lane 5: HCT 116 cell lysate (20 µg/Lane), Lane 6: MCF7 cell lysate (20 µg/Lane), Lane 7: RAW264.7 cell lysate (20 µg/Lane), Lane 8: Mouse heart tissue lysate (40 µg/Lane), Lane 9: Mouse liver tissue lysate (40 µg/Lane), Lane 10: Rat liver tissue lysate (40 µg/Lane), Lane 11: Human liver tissue lysate (40 µg/Lane), Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-FUNDC1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | FUNDC1 Rabbit Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes a protein with a FUN14 superfamily domain. The function of the encoded protein is not known. |
| Immunogen | Recombinant protein within N terminal human FUNDC1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000 IHC:1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | FUNDC1 antibody FUN14 domain-containing protein 1 antibody |
|---|---|
| Molecular Weight(MW) | 17 kDa |
| Cellular Localization | Mitochondrion outer membrane. |
| SwissProt ID | Q8IVP5 |
|---|
WB
Western blot analysis of FUNDC1 on different lysates with Rabbit anti-FUNDC1 antibody at 1/1,000 dilution. Lane 1: 293T cell lysate (20 µg/Lane), Lane 2: Jurkat cell lysate (20 µg/Lane), Lane 3: HepG2 cell lysate (20 µg/Lane), Lane 4: HeLa cell lysate (20 µg/Lane), Lane 5: HCT 116 cell lysate (20 µg/Lane), Lane 6: MCF7 cell lysate (20 µg/Lane), Lane 7: RAW264.7 cell lysate (20 µg/Lane), Lane 8: Mouse heart tissue lysate (40 µg/Lane), Lane 9: Mouse liver tissue lysate (40 µg/Lane), Lane 10: Rat liver tissue lysate (40 µg/Lane), Lane 11: Human liver tissue lysate (40 µg/Lane), Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-FUNDC1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000 IHC:1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
