Application
Fig1: Western blot analysis of AFP on different cell lysates using anti-AFP antibody at 1/1000 dilution. Positive control: Lane 1: HepG2 Lane 2: human lung Lane 3: human liverApplication
Fig2: ICC staining AFP in NCCIT cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining AFP in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-AFP antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of Jurkat cells with AFP antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.| Product Name | AFP |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Afp antibody AFPD antibody Alpha fetoglobulin antibody Alpha fetoprotein antibody Alpha fetoprotein precursor antibody Alpha-1-fetoprotein antibody Alpha-fetoglobulin antibody Alpha-fetoprotein antibody alpha-fetoprotein, Hereditary persistence of, included antibody FETA antibody FETA_HUMAN antibody Hereditary persistence of alpha fetoprotein antibody HPAFP antibody |
| Product description | AFP is a protein normally produced by the fetal liver and is present in the fluid surrounding the fetus (amniotic fluid), and crosses the placenta into the mother's blood. AFP gene expression is regulated by the interactions between steroid hormone receptors and transcriptional factors in separate signal transduction pathways. AFP functions as a binding and transporting ligand and cell growth regulator. Elevated expression level of AFP has been implicated in colorectal, ovarian, pancreatic, testicular, and certain liver cancers. High levels of AFP are also seen in some diseases such as hepatitis and colitis. |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:1,000-1:2,000 ICC:1:200 IHC:1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Afp antibody
AFPD antibody
Alpha fetoglobulin antibody
Alpha fetoprotein antibody
Alpha fetoprotein precursor antibody
Alpha-1-fetoprotein antibody
Alpha-fetoglobulin antibody
Alpha-fetoprotein antibody
alpha-fetoprotein Hereditary persistence of included antibody FETA antibody FETA_HUMAN antibody Hereditary persistence of alpha fetoprotein antibody HPAFP antibody |
|---|---|
| Molecular Weight(MW) | 70 kDa |
| Cellular Localization | Secreted |
| SwissProt ID | P02771 |
|---|
Application
Fig1: Western blot analysis of AFP on different cell lysates using anti-AFP antibody at 1/1000 dilution. Positive control: Lane 1: HepG2 Lane 2: human lung Lane 3: human liver
Application
Fig2: ICC staining AFP in NCCIT cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining AFP in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-AFP antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of Jurkat cells with AFP antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.| Positive Control | NCCIT, HepG2, Jurkat,human liver tissue, human lung tissue, human liver cancer tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:200 IHC:1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
