Application
Fig1: Western blot analysis of BMP2 on different lysates using anti-BMP2 antibody at 1/1,000 dilution.Positive control:Lane 1: Rat kidneyLane 2: Mouse kidneyLane 3: Human kidneyLane 4: HelaApplication
Fig2: ICC staining BMP2 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue).Application
Fig3: ICC staining BMP2 in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue).Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat small intestine tissue using anti-BMP2 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-BMP2 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-BMP2 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of Hela cells with BMP2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.| Product Name | BMP2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BDA2 antibody BMP-2 antibody BMP-2A antibody Bmp2 antibody BMP2_HUMAN antibody BMP2A antibody Bone morphogenetic protein 2 antibody Bone morphogenetic protein 2A antibody |
| Product description | BMP-2 belongs to the TGF-β superfamily of proteins. BMP-2 plays an important role in the development of bone and cartilage. It is involved in the hedgehog pathway, TGF beta signaling pathway, and in cytokine-cytokine receptor interaction. It is involved also in cardiac cell differentiation and epithelial to mesenchymal transition. Implantation of BMP-2 in a collagen sponge induces new bone formation and can be used for the treatment of bony defects, delayed union, and non-union. |
| Immunogen | Synthetic peptide (KLH-coupled) within human BMP2 N terminal. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:1,000 ICC:1:200 IHC:1:200 FC:1:100-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | BDA2 antibody BMP-2 antibody BMP-2A antibody Bmp2 antibody BMP2_HUMAN antibody BMP2A antibody Bone morphogenetic protein 2 antibody Bone morphogenetic protein 2A antibody |
|---|---|
| Molecular Weight(MW) | 75kDa |
| Cellular Localization | Secreted |
Application
Fig1: Western blot analysis of BMP2 on different lysates using anti-BMP2 antibody at 1/1,000 dilution.Positive control:Lane 1: Rat kidneyLane 2: Mouse kidneyLane 3: Human kidneyLane 4: Hela
Application
Fig2: ICC staining BMP2 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue).
Application
Fig3: ICC staining BMP2 in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue).
Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat small intestine tissue using anti-BMP2 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-BMP2 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-BMP2 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Hela cells with BMP2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.| Positive Control | MCF-7, Hela, mouse kidney tissue, human kidney tissue, rat kidney tissue, rat small intestine tissue, mouse small intestine tissue, human breast cancer tissue. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:200 IHC:1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
