Application
Fig1: Western blot analysis of LRP1 on different lysates with Rabbit anti-LRP1 antibody at 1/5,000 dilution. Lane 1: Rat lung tissue lysate, Lane 2: Mouse lung tissue lysate, Lane 3: Human liver tissue lysate, Lysates/proteins at 20 µg/Lane. Predicted band size: 505 kDa Observed band size: 80 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.Application
Fig2: ICC staining LRP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining LRP1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining LRP1 in HUVEC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-LRP1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-LRP1 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-LRP1 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-LRP1 antibody. Counter stained with hematoxylin.Application
Fig9: Flow cytometric analysis of Hela cells with LRP1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | LRP1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human LRP1 aa 4,471-4,520 / 4,544. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:10-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | A2MR antibody Alpha 2 macroglobulin receptor antibody alpha 2MR antibody Alpha-2-macroglobulin receptor antibody APOER antibody Apolipoprotein E receptor antibody APR antibody CD 91 antibody CD91 antibody CD91 antigen antibody IGFBP3R antibody LDL receptor related protein 1 antibody Low density lipoprotein receptor related protein 1 antibody Low density lipoprotein related protein 1 antibody Low-density lipoprotein receptor-related protein 1 intracellular domain antibody LRP 1 antibody LRP 515 antibody LRP 85 antibody LRP antibody LRP ICD antibody LRP-1 antibody LRP-515 antibody LRP-85 antibody Lrp1 antibody LRP1 protein antibody LRP1_HUMAN antibody LRP1A antibody LRP515 antibody LRP85 antibody LRPICD antibody MGC88725 antibody Prolow density lipoprotein receptor related protein 1 antibody TbetaR V/LRP 1/IGFBP 3 receptor antibody TbetaRV/LRP1/IGFBP3 receptor antibody TGFBR 5 antibody TGFBR5 antibody Type V tgf beta receptor antibody |
|---|---|
| Molecular Weight(MW) | 505kDa(Observed band size: 80kDa) |
| Cellular Localization | Cytoplasm, Nucleus, Membrane |
Application
Fig1: Western blot analysis of LRP1 on different lysates with Rabbit anti-LRP1 antibody at 1/5,000 dilution. Lane 1: Rat lung tissue lysate, Lane 2: Mouse lung tissue lysate, Lane 3: Human liver tissue lysate, Lysates/proteins at 20 µg/Lane. Predicted band size: 505 kDa Observed band size: 80 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
Application
Fig2: ICC staining LRP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining LRP1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining LRP1 in HUVEC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-LRP1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-LRP1 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-LRP1 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-LRP1 antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of Hela cells with LRP1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | HUVEC, MCF-7, Hela, HepG2, human liver tissue, mouse liver tissue,mouse brain tissue, human lung tissue |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:10-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
