Application
Fig1: Western blot analysis of Ras on different lysates using anti-Ras antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: 293T Lane 3: Mouse brainApplication
Fig2: Flow cytometric analysis of Hela cells with Ras antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Ras [SA39-05] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C-BAS/HAS antibody c-H-ras antibody C-HA-RAS1 antibody CTLO antibody GTPase HRas antibody GTPase KRas antibody GTPase NRas antibody H-Ras-1 antibody H-RASIDX antibody Ha-Ras antibody HAMSV antibody HRAS antibody HRAS1 antibody K RAS2A antibody K RAS2B antibody K RAS4A antibody K RAS4B antibody K-RAS antibody KRAS antibody KRAS1 antibody KRAS2 antibody N-RAS antibody N-terminally processed antibody NRAS antibody NRAS1 antibody p21ras antibody RASH_HUMAN antibody RASH1 antibody RASK2 antibody Transforming protein p21 antibody v Ha ras Harvey rat sarcoma viral oncogene homolog antibody v Ki ras2 Kirsten rat sarcoma viral oncogene homolog antibody v ras neuroblastoma RAS viral oncogene homolog antibody |
| Product description | Ras superfamily is a protein superfamily of small GTPases, which are all related, to a degree, to the Ras protein subfamily (the key human members of which are KRAS, NRAS, and HRAS). Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway. GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, research studies have shown that in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway. The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein. This antibody is predicted to react with H-Ras, N-Ras and K-Ras. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIPFC |
| WB:1:1,000-1:5,000 FC:1:10-1:100 |
|
| Species Reactivity | HumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Alternative Names | C-BAS/HAS antibody c-H-ras antibody C-HA-RAS1 antibody CTLO antibody GTPase HRas antibody GTPase KRas antibody GTPase NRas antibody H-Ras-1 antibody H-RASIDX antibody Ha-Ras antibody HAMSV antibody HRAS antibody HRAS1 antibody K RAS2A antibody K RAS2B antibody K RAS4A antibody K RAS4B antibody K-RAS antibody KRAS antibody KRAS1 antibody KRAS2 antibody N-RAS antibody N-terminally processed antibody NRAS antibody NRAS1 antibody p21ras antibody RASH_HUMAN antibody RASH1 antibody RASK2 antibody Transforming protein p21 antibody v Ha ras Harvey rat sarcoma viral oncogene homolog antibody v Ki ras2 Kirsten rat sarcoma viral oncogene homolog antibody v ras neuroblastoma RAS viral oncogene homolog antibody |
|---|---|
| Molecular Weight(MW) | 18 kDa |
| Cellular Localization | Cytoplasm, Cell membrane, Golgi apparatus. |
Application
Fig1: Western blot analysis of Ras on different lysates using anti-Ras antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: 293T Lane 3: Mouse brain
Application
Fig2: Flow cytometric analysis of Hela cells with Ras antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | MCF-7, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 FC:1:10-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
