WB
Western blot analysis of p75 NGF Receptor on Neuro-2a cell lysates with Rabbit anti-p75 NGF Receptor antibody at 1/2,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of p75 NGF Receptor on PC-12 cell lysates with Rabbit anti-p75 NGF Receptor antibody at 1/5,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Rabbit anti-p75 NGF Receptor antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse uterus tissue with Rabbit anti-p75 NGF Receptor antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat uterus tissue with Rabbit anti-p75 NGF Receptor antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of PC-12 cells labeling p75 NGF Receptor with Rabbit anti-p75 NGF Receptor antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p75 NGF Receptor antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.IF-F
Species: Mouse, Site: Colon, Sample: Frozen section, Antibody concentration: 1/500, Antigen retrieval: Not required.IF-F
Species: Rat, Site: Colon, Sample: Frozen section, Antibody concentration: 1/500, Antigen retrieval: Not required.FC
Flow cytometric analysis of PC-12 cells labeling p75 NGF Receptor. Cells were fixed and permeabilized. Then stained with the primary antibody (1:200) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).IP
p75 NGF Receptor was immunoprecipitated from 0.2 mg C6 cell lysate with Rabbit anti-p75 NGF Receptor antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-p75 NGF Receptor antibody at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: C6 cell lysate (input), Lane 2: Rabbit anti-p75 NGF Receptor antibody IP in C6 cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-p75 NGF Receptor antibody in C6 cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 20 seconds.| Product Name | p75 NGF Receptor Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The Trk oncogene encodes a membrane-spanning protein tyrosine kinase, gp140Trk, whose expression is restricted in vivo to neurons of the sensory spinal and cranial ganglia of neural crest origin. Nerve growth factor (NGF) stimulates tyrosine phosphorylation of Trk A in neural cell lines and in embryonic dorsal root ganglia. Tyrosine phosphorylation of Trk by NGF is rapid, specific and occurs with picomolar quantities of factor, indicating that the response is mediated by physiological amounts of NGF, suggesting that Trk A participates in the primary signal transduction mechanism of NGF. An additional component of the Trk A receptor complex, NGFR p75, binds to the neurotrophic factors with low affinity but is required for efficient signaling. NGFR p75 accelerates Trk A activation and may recruit downstream effector molecules to the liganded complex. |
| Immunogen | Synthetic peptide within Human p75 NGF Receptor aa 345-394 / 427. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | FCICC/IFIF-FIHCIPWB |
| WB:1:2000-1:5000 IHC:1:200-1:1000 ICC/IF:1:50-1:100 IF-F:1:500-1:1000 FC:1:200 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | CD271 antibody CD271 antigen antibody Gp80 LNGFR antibody Gp80-LNGFR antibody Low affinity nerve growth factor receptor antibody Low affinity neurotrophin receptor p75NTR antibody Low-affinity nerve growth factor receptor antibody Nerve growth factor receptor antibody Nerve growth factor receptor TNFR superfamily member 16 antibody NGF receptor antibody Ngfr antibody p75 ICD antibody p75 Neurotrophin receptor antibody p75 NTR antibody p75(NTR) antibody p75NTR antibody TNFR Superfamily Member 16 antibody TNFRSF16 antibody TNR16_HUMAN antibody Tumor necrosis factor receptor superfamily member 16 antibody |
|---|---|
| Molecular Weight(MW) | 45kDa(Observed band size: 70kDa) |
| Cellular Localization | Membrane |
WB
Western blot analysis of p75 NGF Receptor on Neuro-2a cell lysates with Rabbit anti-p75 NGF Receptor antibody at 1/2,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of p75 NGF Receptor on PC-12 cell lysates with Rabbit anti-p75 NGF Receptor antibody at 1/5,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Rabbit anti-p75 NGF Receptor antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse uterus tissue with Rabbit anti-p75 NGF Receptor antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat uterus tissue with Rabbit anti-p75 NGF Receptor antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of PC-12 cells labeling p75 NGF Receptor with Rabbit anti-p75 NGF Receptor antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p75 NGF Receptor antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
IF-F
Species: Mouse, Site: Colon, Sample: Frozen section, Antibody concentration: 1/500, Antigen retrieval: Not required.
IF-F
Species: Rat, Site: Colon, Sample: Frozen section, Antibody concentration: 1/500, Antigen retrieval: Not required.
FC
Flow cytometric analysis of PC-12 cells labeling p75 NGF Receptor. Cells were fixed and permeabilized. Then stained with the primary antibody (1:200) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
IP
p75 NGF Receptor was immunoprecipitated from 0.2 mg C6 cell lysate with Rabbit anti-p75 NGF Receptor antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-p75 NGF Receptor antibody at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: C6 cell lysate (input), Lane 2: Rabbit anti-p75 NGF Receptor antibody IP in C6 cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-p75 NGF Receptor antibody in C6 cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 20 seconds.| Application Notes | WB:1:2000-1:5000 IHC:1:200-1:1000 ICC/IF:1:50-1:100 IF-F:1:500-1:1000 FC:1:200 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
