Application
Fig1: Western blot analysis of Cytokeratin 19 on MCF-7 cell lysates using anti- Cytokeratin 19 antibody at 1/10,000 dilution.Application
Fig2: ICC staining Cytokeratin 19 in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cytokeratin 19 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 19 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-Cytokeratin 19 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Cytokeratin 19 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of MCF-7 cells with Cytokeratin 19 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | Cytokeratin 19 [SA30-06] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 40 kDa keratin intermediate filament antibody CK 19 antibody CK-19 antibody CK19 antibody Cytokeratin 19 antibody Cytokeratin-19 antibody K19 antibody K1C19_HUMAN antibody K1CS antibody Keratin 19 antibody Keratin type I 40 kD antibody Keratin type I 40kD antibody Keratin type I cytoskeletal 19 antibody Keratin, type I cytoskeletal 19 antibody Keratin, type I, 40 kd antibody Keratin-19 antibody KRT19 antibody MGC15366 antibody |
| Product description | Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells and have been found to be useful markers of tissue differentiation, which is directly applicable to the characterization of malignant tumors. For example, many types of cancer cells express Cytokeratin 19 (CK19), an epithelial cytoskeletal protein within the suprabasal squamous epithelium. Cytokeratin 19 is a specific marker of moderate to severe dysplasia and carcinoma in situ in oral cavity squamous epithelium, and measurement of Cytokeratin 19 may be a useful marker in diagnosing hepatoma. Cytokeratin 19 fragment levels in serum have been documented as a marker for lung cancer. Clinical investigations have suggested that serum CYFRA 21-1, a fragment of Cytokeratin 19, may be among the most useful tumor markers. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:5,000-1:10,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:10-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | 40 kDa keratin intermediate filament antibody
CK 19 antibody
CK-19 antibody
CK19 antibody
Cytokeratin 19 antibody
Cytokeratin-19 antibody
K19 antibody
K1C19_HUMAN antibody
K1CS antibody
Keratin 19 antibody
Keratin type I 40 kD antibody
Keratin type I 40kD antibody
Keratin type I cytoskeletal 19 antibody
Keratin type I cytoskeletal 19 antibody Keratin type I 40 kd antibody Keratin-19 antibody KRT19 antibody MGC15366 antibody |
|---|---|
| Molecular Weight(MW) | 40 kDa |
| Cellular Localization | Cytoplasm |
Application
Fig1: Western blot analysis of Cytokeratin 19 on MCF-7 cell lysates using anti- Cytokeratin 19 antibody at 1/10,000 dilution.
Application
Fig2: ICC staining Cytokeratin 19 in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cytokeratin 19 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 19 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-Cytokeratin 19 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Cytokeratin 19 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of MCF-7 cells with Cytokeratin 19 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | Ags, MCF-7, human liver tissue, mouse liver tissue, human breast carcinoma tissue, human stomach carcinoma tissue. |
|---|---|
| Application Notes | WB:1:5,000-1:10,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:10-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
