Application
Fig1: Western blot analysis of NeuN on human brain lysates using anti-NeuN antibody at 1/1,000 dilution.( NeuN is expressed as two alternate transcripts with apparent SDS-PAGE molecular weight 46 and 48kDa)Application
Fig2: ICC staining NeuN in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NeuN antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-NeuN antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of SH-SY-5Y cells with NeuN antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | NeuN [SR45-07] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ56884 antibody FLJ58356 antibody Fox-1 homolog C antibody fox1 homolog C antibody Fox3 antibody FOX3NeuN antibody hexaribonucleotide binding protein 3 antibody HRNBP3 antibody NEUN antibody neuronal nuclei antibody Rbfox3 antibody RFOX3_HUMAN antibody RNA binding protein fox-1 homolog 3 antibody RNA binding protein, fox 1 homolog (C. elegans) 3 antibody hide |
| Product description | Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, and dentate nucleus neurons. This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS-analysis, NeuN was later identified as the Fox-3 gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator. Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:200-1:500 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | FLJ56884 antibody
FLJ58356 antibody
Fox-1 homolog C antibody
fox1 homolog C antibody
Fox3 antibody
FOX3NeuN antibody
hexaribonucleotide binding protein 3 antibody
HRNBP3 antibody
NEUN antibody
neuronal nuclei antibody
Rbfox3 antibody
RFOX3_HUMAN antibody
RNA binding protein fox-1 homolog 3 antibody
RNA binding protein fox 1 homolog (C. elegans) 3 antibody hide |
|---|---|
| Molecular Weight(MW) | 34 kDa |
| Cellular Localization | Cytoplasm, Nucleus |
Application
Fig1: Western blot analysis of NeuN on human brain lysates using anti-NeuN antibody at 1/1,000 dilution.( NeuN is expressed as two alternate transcripts with apparent SDS-PAGE molecular weight 46 and 48kDa)
Application
Fig2: ICC staining NeuN in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NeuN antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-NeuN antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of SH-SY-5Y cells with NeuN antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | SH-SY-5Y, human brain tissue, mouse brain tissue, mouse cerebellum tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:200-1:500 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
