Application
Fig1: Western blot analysis of DUSP6 on mouse pancreas lysates using anti-DUSP6 antibody at 1/1,000 dilution.Application
Fig2: ICC staining DUSP6 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining DUSP6 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining DUSP6 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-DUSP6 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-DUSP6 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-DUSP6 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-DUSP6 antibody. Counter stained with hematoxylin.Application
Fig9: Flow cytometric analysis of NIH/3T3 cells with DUSP6 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | DUSP6 [SR39-09] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Dual specificity phosphatase 6 antibody Dual specificity phosphatase 6 isoform a antibody Dual specificity protein phosphatase 6 antibody Dual specificity protein phosphatase PYST1 antibody DUS6_HUMAN antibody DUSP 6 antibody DUSP 6a antibody Dusp6 antibody DUSP6a antibody HH19 antibody MAP kinase phosphatase 3 antibody Mitogen activated protein kinase phosphatase 3 antibody Mitogen-activated protein kinase phosphatase 3 antibody MKP 3 antibody MKP-3 antibody MKP3 antibody PYST 1 antibody PYST1 antibody Serine/threonine specific protein phosphatase antibody |
| Product description | Mitogen-activated protein (MAP) kinases are a large class of proteins involved in signal transduction pathways that are activated by a range of stimuli and mediate a number of physiological and pathological changes in the cell. Dual specificity phosphatases (DSPs) are a subclass of the protein tyrosine phosphatase (PTP) gene superfamily, which are selective for dephosphorylating critical phosphothreonine and phosphotyrosine residues within MAP kinases. DSP gene expression is induced by a host of growth factors and/or cellular stresses, thereby negatively regulating MAP kinase superfamily members including MAPK/ERK, SAPK/JNK and p38. The members of the dual-specificity phosphatase protein family include MKP-1/CL100 (3CH134), VHR, PAC1, MKP-2, hVH-3 (B23), hVH-5, MKP-3, MKP-X, and MKP-4. Human MKP-3 maps to chromosome 12q22-q23 and encodes a 381 amino acid protein that specifically inactivates members of the ERK family and is expressed in a variety of tissues with the highest levels in heart and pancreas. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Dual specificity phosphatase 6 antibody Dual specificity phosphatase 6 isoform a antibody Dual specificity protein phosphatase 6 antibody Dual specificity protein phosphatase PYST1 antibody DUS6_HUMAN antibody DUSP 6 antibody DUSP 6a antibody Dusp6 antibody DUSP6a antibody HH19 antibody MAP kinase phosphatase 3 antibody Mitogen activated protein kinase phosphatase 3 antibody Mitogen-activated protein kinase phosphatase 3 antibody MKP 3 antibody MKP-3 antibody MKP3 antibody PYST 1 antibody PYST1 antibody Serine/threonine specific protein phosphatase antibody |
|---|---|
| Molecular Weight(MW) | 42 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of DUSP6 on mouse pancreas lysates using anti-DUSP6 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining DUSP6 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining DUSP6 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining DUSP6 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-DUSP6 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-DUSP6 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-DUSP6 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-DUSP6 antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of NIH/3T3 cells with DUSP6 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | PC-12, NIH/3T3, Hela, mouse pancreas tissue, human gastric carcinoma tissue, human pancreas tissue, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
