Application
Fig1: Western blot analysis of PSD95 on different lysates using anti-PSD95 antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse brain Lane 2: Human brainApplication
Fig2: ICC staining PSD95 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining PSD95 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PSD95 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-PSD95 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse cerebullum tissue using anti-PSD95 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of SH-SY-5Y cells with PSD95 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | PSD95 [SR38-09] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Discs large homolog 4 antibody Disks large homolog 4 antibody DLG 4 antibody Dlg4 antibody DLG4_HUMAN antibody FLJ97752 antibody FLJ98574 antibody Human post synaptic density protein 95 antibody Post synaptic density protein 95 antibody Postsynaptic density protein 95 antibody PSD 95 antibody PSD-95 antibody PSD95 antibody SAP 90 antibody SAP-90 antibody SAP90 antibody Synapse associated protein 90 antibody Synapse-associated protein 90 antibody Tax interaction protein 15 antibody |
| Product description | The Drosophila discs large (dlg) tumor suppressor gene was first identified in Drosophila through genetic analysis of germline mutations. Several mammalian homologs were subsequently identified and categorized into a protein family termed MAGUK (membrane-associated guanylate kinase homolog). Human homologs of dlg include hdlg-1 (rat SAP 97) and NE-dlg (neuronal and endocrine dlg). The rat synaptic protein PSD-95 (also designated SAP 90) also shares homology with these proteins. MAGUKs are localized at the membrane-cytoskeleton interface and contain several distinct domains which suggest a role for these proteins in intracellular signal transduction. Interaction of hdlg-1 and NE-dlg with the tumor suppresor protein APC suggest that MAGUK proteins may also play a role in regulation of growth. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Discs large homolog 4 antibody Disks large homolog 4 antibody DLG 4 antibody Dlg4 antibody DLG4_HUMAN antibody FLJ97752 antibody FLJ98574 antibody Human post synaptic density protein 95 antibody Post synaptic density protein 95 antibody Postsynaptic density protein 95 antibody PSD 95 antibody PSD-95 antibody PSD95 antibody SAP 90 antibody SAP-90 antibody SAP90 antibody Synapse associated protein 90 antibody Synapse-associated protein 90 antibody Tax interaction protein 15 antibody |
|---|---|
| Molecular Weight(MW) | 90 kDa |
| Cellular Localization | Cell membrane, Cell junction |
Application
Fig1: Western blot analysis of PSD95 on different lysates using anti-PSD95 antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse brain Lane 2: Human brain
Application
Fig2: ICC staining PSD95 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining PSD95 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PSD95 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-PSD95 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse cerebullum tissue using anti-PSD95 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of SH-SY-5Y cells with PSD95 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | N2A, SH-SY-5Y, rat brain tissue, mouse cerebullum tissue, rat cerebellum tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
