Application
Fig1: Western blot analysis of IRF1 on different lysates using anti-IRF1 antibody at 1/1,000 dilution. Positive control: Lane 1: PC-12 Lane 2: JurkatApplication
Fig2: ICC staining IRF1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining IRF1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-IRF1 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-IRF1 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Jurkat cells with IRF1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | IRF1 [SR44-08] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Interferon regulatory factor 1 antibody Interferon regulatory factor 1 isoform +I9 antibody Interferon regulatory factor 1 isoform d78 antibody Interferon regulatory factor 1 isoform delta4 antibody Interferon regulatory factor 1 isoform delta7 antibody IRF 1 antibody IRF-1 antibody IRF1 antibody IRF1_HUMAN antibody MAR antibody MAR1 antibody |
| Product description | Interferon regulatory factor-1 (IRF-1) and IRF-2 have been identified as novel DNA-binding factors that function as regulators of both type I interferon (interferon- and ∫) and interferon-inducible genes. The two factors are structurally related, particularly in their N-terminal regions, which confer DNA binding specificity. In addition, both bind to the same sequence within the promoters of interferon- and interferon-∫ genes. IRF-1 functions as an activator of interferon transcription, while IRF-2 binds to the same cis elements and represses IRF-1 action. IRF-1 and IRF-2 have been reported to act in a mutually antagonistic manner in regulating cell growth; overexpression of the repressor IRF-2 leads to cell transformation while concomitant overexpression of IRF-1 causes reversion. IRF-1 and IRF-2 are members of a larger family of DNA binding proteins that includes IRF-3, IRF-4, IRF-5, IRF-6, IRF-7, ISGF-3 p48 (a component of the ISGF-3 complex) and IFN consensus sequence-binding protein (ICSBP). |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Interferon regulatory factor 1 antibody Interferon regulatory factor 1 isoform +I9 antibody Interferon regulatory factor 1 isoform d78 antibody Interferon regulatory factor 1 isoform delta4 antibody Interferon regulatory factor 1 isoform delta7 antibody IRF 1 antibody IRF-1 antibody IRF1 antibody IRF1_HUMAN antibody MAR antibody MAR1 antibody |
|---|---|
| Molecular Weight(MW) | 48 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
Application
Fig1: Western blot analysis of IRF1 on different lysates using anti-IRF1 antibody at 1/1,000 dilution. Positive control: Lane 1: PC-12 Lane 2: Jurkat
Application
Fig2: ICC staining IRF1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining IRF1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-IRF1 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-IRF1 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Jurkat cells with IRF1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | NIH/3T3, PC-12, Hela, Jurkat, human colon cancer tissue, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
