Application
Fig1: Western blot analysis of Rab9 on different lysates using anti-Rab9 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: K562Application
Fig2: ICC staining Rab9 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Rab9 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | Rab9 [SR45-05] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 2410064E05Rik antibody AI195561 antibody DmRab9 antibody Rab 9 antibody RAB 9A antibody RAB9 member RAS oncogene family antibody RAB9A antibody RAB9A member RAS oncogene family antibody RAB9A_HUMAN antibody RAS ASSOCIATED PROTEIN RAB9 antibody Ras related protein Rab 9A antibody Ras-related protein Rab-9A antibody Sid6061p antibody Sid99 antibody |
| Product description | The Ras-related superfamily of guanine nucleotide binding proteins, which includes the R-Ras, Rap, Ral/Rec and Rho/Rab subfamilies exhibit 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles. At least eight members of the Rab subfamily have been identified, each of which is found at a particular stage of a membrane transport pathway. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IF |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 2410064E05Rik antibody AI195561 antibody DmRab9 antibody Rab 9 antibody RAB 9A antibody RAB9 member RAS oncogene family antibody RAB9A antibody RAB9A member RAS oncogene family antibody RAB9A_HUMAN antibody RAS ASSOCIATED PROTEIN RAB9 antibody Ras related protein Rab 9A antibody Ras-related protein Rab-9A antibody Sid6061p antibody Sid99 antibody |
|---|---|
| Molecular Weight(MW) | 23 kDa |
| Cellular Localization | Cell membrane, Endoplasmic reticulum membrane, Golgi apparatus membrane. |
Application
Fig1: Western blot analysis of Rab9 on different lysates using anti-Rab9 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: K562
Application
Fig2: ICC staining Rab9 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Rab9 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | K562, Hela, HepG2. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
