Application
Fig1: Western blot analysis of MEK1/2 on different cell lysates using anti-MEK1/2 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: PC12 Lane 3: NIH/3T3Application
Fig2: ICC staining MEK1/2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining MEK1/2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MEK1/2 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-MEK1/2 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-MEK1/2 antibody. Counter stained with hematoxylin.Application
Fig7:Western blot analysis of MEK1/2 on hybrid fish (crucian-carp) brain tissue lysates using anti-MEK1/2 antibody at 1/500 dilution.| Product Name | MEK1/2 [SR13-07] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AA589381 antibody CFC3 antibody Dual specificity mitogen-activated protein kinase kinase 1 antibody Dual specificity mitogen-activated protein kinase kinase 2 antibody EC 2.7.12.2 antibody ERK activator kinase 1 antibody ERK activator kinase 2 antibody FLJ26075 antibody MAP kinase kinase 1 antibody MAP kinase kinase 2 antibody MAP2K1 antibody MAP2K2 antibody MAPK/ERK kinase 1 antibody MAPK/ERK kinase 2 antibody MAPKK 1 antibody MAPKK1 antibody MAPKK2 antibody MEK 1 antibody MEK1 antibody MEKK1 antibody Mitogen activated protein kinase kinase 1 antibody Mitogen activated protein kinase kinase 2 antibody Mitogen-activated protein kinase kinase 2, p45 antibody MK2 antibody MKK 1 antibody MKK 2 antibody MKK1 antibody MKK2 antibody MP2K1_HUMAN antibody PRKMK 1 antibody PRKMK 2 antibody Prkmk1 antibody Prkmk2 antibody protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1) antibody Protein kinase, mitogen-activated, kinase 1 antibody Protein kinase, mitogen-activated, kinase 2 antibody |
| Product description | A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIP |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Alternative Names | AA589381 antibody
CFC3 antibody
Dual specificity mitogen-activated protein kinase kinase 1 antibody
Dual specificity mitogen-activated protein kinase kinase 2 antibody
EC 2.7.12.2 antibody
ERK activator kinase 1 antibody
ERK activator kinase 2 antibody
FLJ26075 antibody
MAP kinase kinase 1 antibody
MAP kinase kinase 2 antibody
MAP2K1 antibody
MAP2K2 antibody
MAPK/ERK kinase 1 antibody
MAPK/ERK kinase 2 antibody
MAPKK 1 antibody
MAPKK1 antibody
MAPKK2 antibody
MEK 1 antibody
MEK1 antibody
MEKK1 antibody
Mitogen activated protein kinase kinase 1 antibody
Mitogen activated protein kinase kinase 2 antibody
Mitogen-activated protein kinase kinase 2 p45 antibody MK2 antibody MKK 1 antibody MKK 2 antibody MKK1 antibody MKK2 antibody MP2K1_HUMAN antibody PRKMK 1 antibody PRKMK 2 antibody Prkmk1 antibody Prkmk2 antibody protein kinase mitogen-activated kinase 1 (MAP kinase kinase 1) antibody Protein kinase mitogen-activated kinase 1 antibody Protein kinase mitogen-activated kinase 2 antibody |
|---|---|
| Molecular Weight(MW) | 44 kDa |
| Cellular Localization | Cytoplasm, Nucleus, Membrane, Mitochondrion |
Application
Fig1: Western blot analysis of MEK1/2 on different cell lysates using anti-MEK1/2 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: PC12 Lane 3: NIH/3T3
Application
Fig2: ICC staining MEK1/2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining MEK1/2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MEK1/2 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-MEK1/2 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-MEK1/2 antibody. Counter stained with hematoxylin.
Application
Fig7:Western blot analysis of MEK1/2 on hybrid fish (crucian-carp) brain tissue lysates using anti-MEK1/2 antibody at 1/500 dilution.| Positive Control | PC12, NIH/3T3, A549, HepG2, human kidney tissue, mouse kidney tissue, mouse lung tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
