Application
Fig1: Western blot analysis of Scavenging Receptor SR-BI on different lysates with Rabbit anti-Scavenging Receptor SR-BI antibody at 1/2,000 dilution. Lane 1: HepG2 cell lysate (15 µg/Lane), Lane 2: Human liver tissue lysate (20 µg/Lane), Lane 3: Mouse liver tissue lysate (20 µg/Lane), Lane 4: Rat liver tissue lysate (20 µg/Lane), Exposure time: 3 minutes 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.Application
Fig2: ICC staining Scavenging Receptor SR-BI in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Scavenging Receptor SR-BI in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Scavenging Receptor SR-BI antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Scavenging Receptor SR-BI antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Scavenging Receptor SR-BI antibody. Counter stained with hematoxylin.| Product Name | Scavenging Receptor SR-BI Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The macrophage class A scavenger receptors (SR-A) type I and II mediate the uptake of modified low density lipoprotein (LDL), while the scavenger receptor class B type 1 (SR-B1) mediates the selective uptake of cholesterol and cholesterol esters (CE) from HDLs into cells. SREC, Ox-LDL-R1, SR-A and SR-B1 may all be involved in the early development of atherosclerosis. SR-B1, an integral membrane protein, acts as a receptor for various ligands, including apoptotic cells, cholesterol ester, phospholipids, lipoproteins and phosphatidyl-serine. SR-B1, which may be involved in phagocytosis of apoptotic cells, enables the movement of cholesterol between the cell surface and extracellular donors and acceptors. Although it is widely expressed, SR-B1 localizes primarily to cholesterol and sphingomyelin-enriched domains within the plasma membrane, called caveolae. |
| Immunogen | Synthetic peptide within Human SRB1 aa 81-130 / 552. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000 ICC/IF:1:50 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | CD36 and LIMPII analogous 1 antibody CD36 antibody CD36 Antigen like 1 antibody CD36 antigen-like 1 antibody CD36L1 antibody CLA 1 antibody CLA-1 antibody CLA1 antibody Collagen type I receptor antibody HDLQTL6 antibody MGC138242 antibody SCARB1 antibody Scavebger Receptor Class B Member 1 antibody Scavenger receptor class B member 1 antibody Scavenger Receptor Class B Type 1 antibody SCRB1_HUMAN antibody SR BI antibody SR-BI antibody SRB1 antibody SRBI antibody Thrombospondin receptor like 1 antibody thrombospondin receptor-like 1 antibody |
|---|---|
| Molecular Weight(MW) | 61 kDa(Observed band size: 75 kDa) |
| Cellular Localization | Cell membrane, Membrane. |
Application
Fig1: Western blot analysis of Scavenging Receptor SR-BI on different lysates with Rabbit anti-Scavenging Receptor SR-BI antibody at 1/2,000 dilution. Lane 1: HepG2 cell lysate (15 µg/Lane), Lane 2: Human liver tissue lysate (20 µg/Lane), Lane 3: Mouse liver tissue lysate (20 µg/Lane), Lane 4: Rat liver tissue lysate (20 µg/Lane), Exposure time: 3 minutes 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Application
Fig2: ICC staining Scavenging Receptor SR-BI in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Scavenging Receptor SR-BI in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Scavenging Receptor SR-BI antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Scavenging Receptor SR-BI antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Scavenging Receptor SR-BI antibody. Counter stained with hematoxylin.| Positive Control | CRC, PC-12, human liver tissue, mouse liver tissue, human spleen tissue. |
|---|---|
| Application Notes | WB:1:2000 ICC/IF:1:50 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
