Application
Fig1: Western blot analysis of Histone H2A(acetyl K9) on different cell lysates using anti-Histone H2A(acetyl K9) antibody at 1/500 dilution. Positive control: Lane 1: HeLa treated with 500 ng/ml Trichostatin A for 4 hours whole cell lysates Lane 2: Untreated HeLa whole cell lysatesApplication
Fig2: ICC staining Histone H2A(acetyl K9) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Histone H2A(acetyl K9) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Histone H2A(acetyl K9) in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.Application
Fig10: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.Application
Fig11: Immunohistochemical analysis of paraffin-embedded mouse thyroid tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.| Product Name | Histone H2A(acetyl K9) [SR4-15] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | H2A antibody H2A1B_HUMAN antibody H2AFM antibody HIST1H2A antibody HIST1H2AE antibody Histone H2A type 1-B/E antibody Histone H2A.2 antibody Histone H2A/a antibody Histone H2A/m antibody |
| Product description | Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation. |
| Immunogen | recombinant protein |
| Modification | acetyl K9 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | H2A antibody H2A1B_HUMAN antibody H2AFM antibody HIST1H2A antibody HIST1H2AE antibody Histone H2A type 1-B/E antibody Histone H2A.2 antibody Histone H2A/a antibody Histone H2A/m antibody |
|---|---|
| Molecular Weight(MW) | 14 kDa |
| Cellular Localization | Nucleus, Chromosome. |
Application
Fig1: Western blot analysis of Histone H2A(acetyl K9) on different cell lysates using anti-Histone H2A(acetyl K9) antibody at 1/500 dilution. Positive control: Lane 1: HeLa treated with 500 ng/ml Trichostatin A for 4 hours whole cell lysates Lane 2: Untreated HeLa whole cell lysates
Application
Fig2: ICC staining Histone H2A(acetyl K9) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Histone H2A(acetyl K9) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Histone H2A(acetyl K9) in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.
Application
Fig10: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.
Application
Fig11: Immunohistochemical analysis of paraffin-embedded mouse thyroid tissue using anti-Histone H2A(acetyl K9) antibody. Counter stained with hematoxylin.| Positive Control | A549, MCF-7, Hela, human breast carcinoma tissue, human tonsil tissue, mouse testis tissue, human lung carcinoma tissue, mouse colon tissue, human colon cancer tissue, mouse thyroid tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
