Application
Fig1: Western blot analysis of Firefly Luciferase on luciferase recombinant protein lysate using anti-Firefly Luciferase antibody at 1/1,000 dilution.| Product Name | Firefly Luciferase [SR03-86] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ec 1.13.12.7 antibody Firefly antibody Luciferase antibody Luciferin 4 monooxygenase antibody |
| Product description | Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIF |
| WB:1:500-1:2,000 |
|
| Species Reactivity | Firefly |
| Concentration | 1mg/ml |
| Alternative Names | ec 1.13.12.7 antibody Firefly antibody Luciferase antibody Luciferin 4 monooxygenase antibody |
|---|---|
| Cellular Localization | Peroxisome. |
| SwissProt ID | P08659 |
|---|
Application
Fig1: Western blot analysis of Firefly Luciferase on luciferase recombinant protein lysate using anti-Firefly Luciferase antibody at 1/1,000 dilution.| Positive Control | Luciferase recombinant protein. |
|---|---|
| Application Notes | WB:1:500-1:2,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
