WB
Western blot analysis of Maltose Binding Protein on recombinant MBP-tag protein lysates using anti-Maltose Binding Protein antibody at 1/1,000 dilution.IP
MBP tag was immunoprecipitated in 2µg MBP Tag fusion protein lysate with anti-Maltose Binding Protein antibody at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate usinganti-Maltose Binding Protein antibody at 1/1,000 dilution. Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 60 mins at room temperature. Lane 1: MBP Tag fusion protein lysate (input). Lane 2: anti-Maltose Binding Protein antibody IP in MBP Tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST.| Product Name | Maltose Binding Protein Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors frequently encode hybrid fusion proteins consisting in part of prokaryotic and in part, eukaryotic specified proteins. One such system utilizes maltose binding protein (MBP), the 370 amino acid product of the E. coli mal E gene. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. Expression systems utilizing the MBP fusion tag include pCG-806fx and pMal vectors. |
| Immunogen | Synthetic peptide within Escherichia coli MBP aa 331-380 / 396. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | IPWB |
| WB:1:1000-1:5000 IP:2-5 µg/ml |
|
| Species Reactivity | All |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | ECK4026 antibody JW3994 antibody Mal E antibody MalE antibody malJ antibody Maltodextrin binding protein antibody Maltose ABC transporter periplasmic protein antibody Maltose binding periplasmic protein antibody MMBP antibody Periplasmic maltose binding protein antibody |
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WB
Western blot analysis of Maltose Binding Protein on recombinant MBP-tag protein lysates using anti-Maltose Binding Protein antibody at 1/1,000 dilution.
IP
MBP tag was immunoprecipitated in 2µg MBP Tag fusion protein lysate with anti-Maltose Binding Protein antibody at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate usinganti-Maltose Binding Protein antibody at 1/1,000 dilution. Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 60 mins at room temperature. Lane 1: MBP Tag fusion protein lysate (input). Lane 2: anti-Maltose Binding Protein antibody IP in MBP Tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST.| Positive Control | Recombinant MBP-tag protein. |
|---|---|
| Application Notes | WB:1:1000-1:5000 IP:2-5 µg/ml |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
