Application
Fig1: Western blot analysis of Caveolin-1 on different lysates with Rabbit anti-Caveolin-1 antibody at 1/1,000 dilution. Lane 1: A549 cell lysate (10 µg/Lane), Lane 2: A431 cell lysate (10 µg/Lane), Lane 3: Human lung tissue lysate (40 µg/Lane), Lane 4: Mouse lung tissue lysate (40 µg/Lane), Lane 5: Mouse heart tissue lysate (40 µg/Lane), Lane 6: Rat lung tissue lysate (40 µg/Lane), Lane 7: Rat heart tissue lysate (40 µg/Lane), Exposure time: Lane 1-4: 6 seconds; Lane 5-7: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.Application
Fig2: ICC staining Caveolin-1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Caveolin-1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Caveolin-1 in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Caveolin-1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Caveolin-1 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Caveolin-1 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Caveolin-1 antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Caveolin-1 antibody. Counter stained with hematoxylin.| Product Name | Caveolin-1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Caveolae (also known as plasmalemmal vesicles) are 50-100 nM flask-shaped membranes that represent a subcompartment of the plasma membrane. On the basis of morphological studies, caveolae have been implicated to function in the transcytosis of various macromolecules (including LDL) across capillary endothelial cells, uptake of small molecules via potocytosis and the compartmentalization of certain signaling molecules including G protein-coupled receptors. Three proteins, caveolin-1, caveolin-2 and caveolin-3, have been identified as principal components of caveolae. Two forms of caveolin-1, designated alpha and beta, share a distinct but overlapping cellular distribution and differ by an amino terminal 31 amino acid sequence which is absent from the beta isoform. Caveolin-1 shares 31% identity with caveolin-2 and 65% identity with caveolin-3 at the amino acid level. Functionally, the three proteins differ in their interactions with heterotrimeric G protein isoforms. |
| Immunogen | Synthetic peptide within Human Caveolin-1 aa 129-178 / 178. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:5000 ICC/IF:1:50-1:200 IHC:1:50-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | BSCL3 antibody
CAV antibody
CAV1 antibody
CAV1_HUMAN antibody
caveolae protein 22 kD antibody caveolin 1 alpha isoform antibody caveolin 1 beta isoform antibody Caveolin 1 caveolae protein 22kDa antibody Caveolin-1 antibody Caveolin1 antibody cell growth-inhibiting protein 32 antibody CGL3 antibody LCCNS antibody MSTP085 antibody OTTHUMP00000025031 antibody PPH3 antibody VIP 21 antibody VIP21 antibody |
|---|---|
| Molecular Weight(MW) | 20kDa(Observed band size: 20/18kDa) |
| Cellular Localization | Golgi apparatus membrane, Cell membrane, Membrane. |
| SwissProt ID | Q03135 |
|---|
Application
Fig1: Western blot analysis of Caveolin-1 on different lysates with Rabbit anti-Caveolin-1 antibody at 1/1,000 dilution. Lane 1: A549 cell lysate (10 µg/Lane), Lane 2: A431 cell lysate (10 µg/Lane), Lane 3: Human lung tissue lysate (40 µg/Lane), Lane 4: Mouse lung tissue lysate (40 µg/Lane), Lane 5: Mouse heart tissue lysate (40 µg/Lane), Lane 6: Rat lung tissue lysate (40 µg/Lane), Lane 7: Rat heart tissue lysate (40 µg/Lane), Exposure time: Lane 1-4: 6 seconds; Lane 5-7: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Application
Fig2: ICC staining Caveolin-1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Caveolin-1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Caveolin-1 in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Caveolin-1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Caveolin-1 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Caveolin-1 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Caveolin-1 antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Caveolin-1 antibody. Counter stained with hematoxylin.| Application Notes | WB:1:500-1:5000 ICC/IF:1:50-1:200 IHC:1:50-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
