WB
Western blot analysis of ERG on Jurkat cell lysates with Rabbit anti-ERG antibody at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of ERG on different lysates with Rabbit anti-ERG antibody at 1/2,000 dilution. Lane 1: Mouse lung tissue lysate, Lane 2: Mouse spleen tissue lysate, Lane 3: Rat brain tissue lysate, Lane 4: Rat lung tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-ERG antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-ERG antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-ERG antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | ERG Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2, Erg-1–3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF. Members of the Ets gene family exhibit varied patterns of tissue expression, and share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. This conserved domain is essential for Ets-1 binding to DNA and is likely to be responsible for the DNA binding activity of all members of the Ets gene family. Several of these proteins have been shown to recognize similar motifs in DNA that share a centrally located 5'-GGAA-3' element. Erg genes encode for multiple proteins due to alternative splicing and alternative usage of initiation codons. |
| Immunogen | Synthetic peptide within Human ERG aa 430-479 / 479. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | IHCWB |
| WB:1:2000-1:5000 IHC:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | Avian erythroblastosis virus E-26 (v-ets) oncogene related antibody
D030036I24Rik antibody
Erg 3 antibody
Erg antibody
ERG/EWS fusion gene included antibody ERG/FUS fusion gene included antibody ERG/TMPSSR2 fusion gene included antibody ERG_HUMAN antibody ERG1 included antibody ERG2 included antibody ets related antibody ETS-related gene antibody KCNH2 antibody Oncogene ERG antibody p55 antibody TMPRSS2/ERG fusion antibody transcriptional regulator ERG (transforming protein ERG) antibody Transcriptional regulator ERG antibody Transforming protein ERG antibody v ets avian erythroblastosis virus E26 oncogene antibody v ets avian erythroblastosis virus E26 oncogene related antibody v ets erythroblastosis virus E26 oncogene homolog antibody v ets erythroblastosis virus E26 oncogene like antibody v ets erythroblastosis virus E26 oncogene like isoform 2 antibody v-ets erythroblastosis virus E26 oncogene antibody v-ets erythroblastosis virus E26 oncogene homolog (avian) antibody V-ets erythroblastosis virus E26 oncogene like (Avian) isoform CRA_e antibody |
|---|---|
| Molecular Weight(MW) | 54 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
WB
Western blot analysis of ERG on Jurkat cell lysates with Rabbit anti-ERG antibody at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of ERG on different lysates with Rabbit anti-ERG antibody at 1/2,000 dilution. Lane 1: Mouse lung tissue lysate, Lane 2: Mouse spleen tissue lysate, Lane 3: Rat brain tissue lysate, Lane 4: Rat lung tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-ERG antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-ERG antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-ERG antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:2000-1:5000 IHC:1:200-1:1000 |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
