Application
Fig1: ICC staining VEGF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining VEGF in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining VEGF in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining VEGF in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-VEGF antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcimoma tissue using anti-VEGF antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-VEGF antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-VEGF antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-VEGF antibody. Counter stained with hematoxylin.Application
Fig10: Flow cytometric analysis of Hela cells with VEGF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Product Name | VEGF [SP07-01] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Folliculostellate cell-derived growth factor antibody Glioma-derived endothelial cell mitogen antibody MGC70609 antibody MVCD1 antibody Vascular endothelial growth factor A antibody vascular endothelial growth factor A121 antibody vascular endothelial growth factor A165 antibody vascular endothelial growth factor antibody Vascular permeability factor antibody VEGF A antibody Vegf antibody VEGF-A antibody VEGF120 antibody Vegfa antibody VEGFA_HUMAN antibody VPF antibody |
| Product description | The onset of angiogenesis is believed to be an early event in tumorigenesis and may facilitate tumor progression and metastasis. Several growth factors with angiogenic activity have been described. These include fibroblast growth factors (FGFs), platelet derived growth factor (PDGF) and vascular endothelial growth factor (VEGF). VEGF is a dimeric glycoprotein with structural homology to PDGF. Several variants of VEGF have been described that arise by alternative mRNA splicing. It has been speculated that VEGF may function as a tumor angiogenesis factor in vivo because the expression pattern of VEGF is consistent with a role in embryonic angiogenesis. VEGF mRNA is formed in some primary tumors, VEGF is produced by tumor cell lines in vitro and VEGF mitogenic activity appears to be restricted to endothelial cells. A member of the PDGF receptor family, Flt, has been identified as a high-affinity receptor for VEGF. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | ICC/IFIHCIPFC |
| ICC:1:50-1:200 IHC:1:50-1:200: FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Folliculostellate cell-derived growth factor antibody Glioma-derived endothelial cell mitogen antibody MGC70609 antibody MVCD1 antibody Vascular endothelial growth factor A antibody vascular endothelial growth factor A121 antibody vascular endothelial growth factor A165 antibody vascular endothelial growth factor antibody Vascular permeability factor antibody VEGF A antibody Vegf antibody VEGF-A antibody VEGF120 antibody Vegfa antibody VEGFA_HUMAN antibody VPF antibody |
|---|---|
| Molecular Weight(MW) | 43 kDa |
| Cellular Localization | Secreted. |
Application
Fig1: ICC staining VEGF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining VEGF in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining VEGF in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining VEGF in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-VEGF antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcimoma tissue using anti-VEGF antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-VEGF antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-VEGF antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-VEGF antibody. Counter stained with hematoxylin.
Application
Fig10: Flow cytometric analysis of Hela cells with VEGF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | SHG-44, MCF-7, Hela, SH-SY-5Y, mouse kidney tissue, human colon cancer tissue, mouse cerebellum tissue, human breast carcimoma tissue, human kidney tissue. |
|---|---|
| Application Notes | ICC:1:50-1:200 IHC:1:50-1:200: FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
