WB
Western blot analysis of ALDH1A1 on different lysates with Rabbit anti-ALDH1A1 antibody at 1/2,000 dilution. Lane 1: mouse lung tissue lysate, Lane 2: human liver tissue lysate, Lane 3: human kidney tissue lysate, Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rabbit anti-ALDH1A1 antibody at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-F
Species: Mouse, Site: Striatum, Sample: Frozen section, Antibody concentration: 1:200, Antigen retrieval: Recommend. The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature.| Product Name | ALDH1A1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Aldehyde dehydrogenases (ALDHs) mediate NADP+-dependent oxidation of aldehydes into acids during the detoxification of alcohol-derived acetaldehyde; metabolism of corticosteroids, biogenic amines and neurotransmitters; and lipid peroxidation. ALDH1A1, also designated retinal dehydrogenase 1 (RalDH1 or RALDH1), aldehyde dehydrogenase family 1 member A1, aldehyde dehydrogenase cytosolic, ALDHII, ALDH-E1 or ALDH E1, is a retinal dehydrogenase that participates in the biosynthesis of retinoic acid (RA). There are two major liver isoforms of ALDH1 that can localize to cytosolic or mitochondrial space. The ALDH1A2 (RALDH2, RALDH2-T) gene produces three different transcripts and also catalyzes the synthesis of RA from retinaldehyde. ALDH1A3 (ALDH6, RALDH3, ALDH1A6) is a 37 kb gene that consists of 13 exons and produces a major transcript of approximately 3.5 kb most abundant in salivary gland, stomach and kidney. ALDH3A1 (stomach type, ALDH3, ALDHIII) forms a cytoplasmic homodimer that preferentially oxidizes aromatic aldehyde substrates. ALDH genes upregulate as a part of the oxidative stress response, and appear to be abundant in certain tumors that have an accelerated metabolism toward chemotherapy agents. |
| Immunogen | Synthetic peptide within human ALDH1A1 aa 300-360. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | IF-FIHCIPWB |
| WB:1:1000-1:2000 IHC:1:100-1:200 IF-F:1:200 IP:Use at an assay dependent concentration. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | Acetaldehyde dehydrogenase 1 antibody
AHD2 antibody
AL1A1_HUMAN antibody
ALDC antibody
Aldehyde dehydrogenase 1 family member A1 antibody
Aldehyde dehydrogenase 1 soluble antibody
Aldehyde dehydrogenase 1A1 antibody
Aldehyde dehydrogenase antibody
Aldehyde dehydrogenase cytosolic antibody
Aldehyde dehydrogenase family 1 member A1 antibody
Aldehyde dehydrogenase liver cytosolic antibody
ALDH 1 antibody
ALDH 1A1 antibody
ALDH class 1 antibody
ALDH liver cytosolic antibody ALDH-E1 antibody ALDH1 A1 antibody ALDH1 antibody ALDH11 antibody ALDH1A1 antibody ALHDII antibody cytosolic antibody epididymis luminal protein 12 antibody epididymis luminal protein 9 antibody epididymis secretory sperm binding protein Li 53e antibody HEL-S-53e antibody MGC2318 antibody PUMB1 antibody RALDH 1 antibody RalDH1 antibody Retinal dehydrogenase 1 antibody |
|---|---|
| Molecular Weight(MW) | 55 kDa |
| Cellular Localization | Cytoplasm,Cell projection. |
WB
Western blot analysis of ALDH1A1 on different lysates with Rabbit anti-ALDH1A1 antibody at 1/2,000 dilution. Lane 1: mouse lung tissue lysate, Lane 2: human liver tissue lysate, Lane 3: human kidney tissue lysate, Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rabbit anti-ALDH1A1 antibody at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-F
Species: Mouse, Site: Striatum, Sample: Frozen section, Antibody concentration: 1:200, Antigen retrieval: Recommend. The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature.| Application Notes | WB:1:1000-1:2000 IHC:1:100-1:200 IF-F:1:200 IP:Use at an assay dependent concentration. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
