Application
Fig1: Western blot analysis of STAT3 on MCF-7 cells lysates using anti-STAT3 antibody at 1/1,000 dilution.Application
Fig2: ICC staining STAT3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining STAT3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining STAT3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-STAT3 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-STAT3 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-STAT3 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-STAT3 antibody. Counter stained with hematoxylin.| Product Name | STAT3 [SY34-01] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 1110034C02Rik antibody Acute Phase Response Factor antibody Acute-phase response factor antibody ADMIO antibody APRF antibody AW109958 antibody DNA binding protein APRF antibody FLJ20882 antibody HIES antibody MGC16063 antibody Signal transducer and activator of transcription 3 (acute phase response factor) antibody Signal transducer and activator of transcription 3 antibody STAT 3 antibody Stat3 antibody STAT3_HUMAN antibody |
| Product description | Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of JAK kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by Prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:1,000-1:2,000 ICC:1:100-1:500 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 1110034C02Rik antibody Acute Phase Response Factor antibody Acute-phase response factor antibody ADMIO antibody APRF antibody AW109958 antibody DNA binding protein APRF antibody FLJ20882 antibody HIES antibody MGC16063 antibody Signal transducer and activator of transcription 3 (acute phase response factor) antibody Signal transducer and activator of transcription 3 antibody STAT 3 antibody Stat3 antibody STAT3_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 88 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
Application
Fig1: Western blot analysis of STAT3 on MCF-7 cells lysates using anti-STAT3 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining STAT3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining STAT3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining STAT3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-STAT3 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-STAT3 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-STAT3 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-STAT3 antibody. Counter stained with hematoxylin.| Positive Control | A549, MCF-7, Hela, HepG2, human breast carcinoma tissue, human gastric carcinoma tissue, mouse brain tissue, mouse pancreas tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:100-1:500 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
