Application
Fig1: ICC staining Musashi 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining Musashi 1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Musashi 1 antibody. Counter stained with hematoxylin.Application
Fig4: Flow cytometric analysis of Hela cells with Musashi 1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Musashi 1 [SJ201] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Msi 1 antibody Msi1 antibody MSI1H_HUMAN antibody Musashi homolog 1 antibody Musashi-1 antibody Musashi1 antibody RNA binding protein Musashi homolog 1 antibody RNA-binding protein Musashi homolog 1 antibody |
| Product description | Musashi1 (Msi1) is an RNA-binding protein expressed in neural progenitor cells and neural stem cells. Msi1 is the mammalian homolog of Drosophila Musashi. The gene encoding human Msi1 encodes a 362 amino acid protein. In murine embryonic neural progenitor cells, Msi1 localizes to the cytoplasm and is downregulated during differentiation. Msi1 binds to NUMB, which encodes a membrane-associated antagonist of Notch signaling. Msi1 appears to function in the proliferation and maintenance of stem cell populations of the central nervous system. In addition to its usefulness as a marker for neural progenitor cells in normal human brains, Msi1 is also a marker for human gliomas. In rats, Msi1 is expressed in Sertoli cells of the testis and granulosa cells of the ovary. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Msi 1 antibody Msi1 antibody MSI1H_HUMAN antibody Musashi homolog 1 antibody Musashi-1 antibody Musashi1 antibody RNA binding protein Musashi homolog 1 antibody RNA-binding protein Musashi homolog 1 antibody |
|---|---|
| Molecular Weight(MW) | 39 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
| SwissProt ID | Q07954 |
|---|
Application
Fig1: ICC staining Musashi 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining Musashi 1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Musashi 1 antibody. Counter stained with hematoxylin.
Application
Fig4: Flow cytometric analysis of Hela cells with Musashi 1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | A549, Hela, human pancreas tissue. |
|---|---|
| Application Notes | WB:1:1000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
