Application
Fig1: Western blot analysis of CD3 epsilon on different lysates using anti-CD3 epsilon antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: Human thymusApplication
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD3 epsilon antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD3 epsilon antibody. Counter stained with hematoxylin.Application
Fig4: Flow cytometric analysis of Jurkat cells with CD3 epsilon antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Product Name | CD3 epsilon [SY0239] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD3 epsilon antibody CD3e antibody CD3e antigen epsilon polypeptide (TiT3 complex) antibody CD3E antigen epsilon polypeptide antibody CD3E antigen, epsilon subunit antibody CD3e molecule epsilon antibody CD3e molecule, epsilon (CD3 TCR complex) antibody CD3e molecule, epsilon (CD3-TCR complex) antibody CD3E_HUMAN antibody IMD18 antibody T cell antigen receptor complex epsilon subunit of T3 antibody T cell surface antigen T3/Leu 4 epsilon chain antibody T cell surface glycoprotein CD3 epsilon chain antibody T-cell surface antigen T3/Leu-4 epsilon chain antibody T-cell surface glycoprotein CD3 epsilon chain antibody T3E antibody TCRE antibody |
| Product description | The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. Much of this signaling process can be attributed to a multisubunit complex of proteins that associates directly with the TCR. This complex has been designated CD3 (cluster of differentiation 3). CD3 is composed of four polypeptides: ζ, γ, ε and δ. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM). Engagement of TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecules such as ZAP-70 and p85 subunit of PI-3 kinase. TCR ligation also induces a conformational change in CD3ε, such that a proline region is exposed and then associates with the adaptor protein Nck. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCIPFC |
| WB:1:1,000-1:2,000 IHC:1:50-1:200: FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CD3 epsilon antibody
CD3e antibody
CD3e antigen epsilon polypeptide (TiT3 complex) antibody
CD3E antigen epsilon polypeptide antibody
CD3E antigen epsilon subunit antibody CD3e molecule epsilon antibody CD3e molecule epsilon (CD3 TCR complex) antibody CD3e molecule epsilon (CD3-TCR complex) antibody CD3E_HUMAN antibody IMD18 antibody T cell antigen receptor complex epsilon subunit of T3 antibody T cell surface antigen T3/Leu 4 epsilon chain antibody T cell surface glycoprotein CD3 epsilon chain antibody T-cell surface antigen T3/Leu-4 epsilon chain antibody T-cell surface glycoprotein CD3 epsilon chain antibody T3E antibody TCRE antibody |
|---|---|
| Molecular Weight(MW) | 23 kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | P07766 |
|---|
Application
Fig1: Western blot analysis of CD3 epsilon on different lysates using anti-CD3 epsilon antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: Human thymus
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD3 epsilon antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD3 epsilon antibody. Counter stained with hematoxylin.
Application
Fig4: Flow cytometric analysis of Jurkat cells with CD3 epsilon antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | Jurkat, human tonsil tissue, human thymus tissue, human spleen tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 IHC:1:50-1:200: FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
