WB
Western blot analysis of N Cadherin on different lysates with Rabbit anti-N Cadherin antibody at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: 293T cell lysate, Lane 2: A549 cell lysate, Lane 3: HeLa cell lysate, Lane 4: A-172 cell lysate, Lane 5: MCF7 cell lysate (negative), Lane 6: C2C12 cell lysate, Lane 7: C6 cell lysate, Lysates/proteins at 15 µg/Lane. Exposure time: 2 minutes 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-N Cadherin antibody at 1/40,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/40,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-N Cadherin antibody at 1/40,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/40,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-F
Species: Mouse, Site: Liver, Sample: Frozen section, Antibody concentration: 1:500, Antigen retrieval: Recommend. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.| Product Name | N Cadherin Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The most distal of these cadherins is thought to be responsible for binding specificity, transmembrane domains and carboxy-terminal intracellular domains. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as b-catenin, to regulate cadherin function. Members of this family of adhesion proteins include rat cadherin K (and its human homolog, cadherin-6), R-cadherin, B-cadherin, E/P cadherin and cadherin-5. |
| Immunogen | Synthetic peptide within Human N Cadherin aa 161-210 / 906. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | IF-FIHCWB |
| WB:1:5000-1:20000 IHC:1:10000-1:40000 IF-F:1:500-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | CADH2_HUMAN antibody
Cadherin 2 antibody
Cadherin 2 N cadherin neuronal antibody
Cadherin 2 type 1 antibody
Cadherin 2 type 1 N cadherin neuronal antibody
Cadherin 2 type 1 N-cadherin (neuronal) antibody Cadherin-2 antibody Cadherin2 antibody Calcium dependent adhesion protein neuronal antibody CD325 antibody CD325 antigen antibody CDH2 antibody CDHN antibody CDw325 antibody CDw325 antigen antibody N cadherin 1 antibody N-cadherin antibody NCAD antibody Neural cadherin antibody OTTHUMP00000066304 antibody OTTHUMP00000067378 antibody |
|---|---|
| Molecular Weight(MW) | 100kDa(Observed band size: 140-150kDa) |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of N Cadherin on different lysates with Rabbit anti-N Cadherin antibody at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: 293T cell lysate, Lane 2: A549 cell lysate, Lane 3: HeLa cell lysate, Lane 4: A-172 cell lysate, Lane 5: MCF7 cell lysate (negative), Lane 6: C2C12 cell lysate, Lane 7: C6 cell lysate, Lysates/proteins at 15 µg/Lane. Exposure time: 2 minutes 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-N Cadherin antibody at 1/40,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/40,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-N Cadherin antibody at 1/40,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/40,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-F
Species: Mouse, Site: Liver, Sample: Frozen section, Antibody concentration: 1:500, Antigen retrieval: Recommend. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.| Application Notes | WB:1:5000-1:20000 IHC:1:10000-1:40000 IF-F:1:500-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
