Application
Fig1: Western blot analysis of Phospho-STAT3(S727) on different lysates using anti-Phospho-STAT3(S727) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3Application
Fig2: ICC staining Phospho-STAT3(S727) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Phospho-STAT3(S727) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Phospho-STAT3(S727) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Phospho-STAT3(S727) antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Phospho-STAT3(S727) antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Phospho-STAT3(S727) antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Phospho-STAT3(S727) antibody. Counter stained with hematoxylin.| Product Name | Phospho-STAT3(S727) [SY24-09] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 1110034C02Rik antibody Acute Phase Response Factor antibody Acute-phase response factor antibody ADMIO antibody APRF antibody AW109958 antibody DNA binding protein APRF antibody FLJ20882 antibody HIES antibody MGC16063 antibody Signal transducer and activator of transcription 3 (acute phase response factor) antibody Signal transducer and activator of transcription 3 antibody STAT 3 antibody Stat3 antibody STAT3_HUMAN antibody |
| Product description | Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of JAK kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by Prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways. |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser727 of human STAT3. |
| Modification | p-S727 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIP |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanRatMouse |
| Concentration | 1mg/ml |
| Alternative Names | 1110034C02Rik antibody Acute Phase Response Factor antibody Acute-phase response factor antibody ADMIO antibody APRF antibody AW109958 antibody DNA binding protein APRF antibody FLJ20882 antibody HIES antibody MGC16063 antibody Signal transducer and activator of transcription 3 (acute phase response factor) antibody Signal transducer and activator of transcription 3 antibody STAT 3 antibody Stat3 antibody STAT3_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 88 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
Application
Fig1: Western blot analysis of Phospho-STAT3(S727) on different lysates using anti-Phospho-STAT3(S727) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3
Application
Fig2: ICC staining Phospho-STAT3(S727) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Phospho-STAT3(S727) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Phospho-STAT3(S727) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Phospho-STAT3(S727) antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Phospho-STAT3(S727) antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Phospho-STAT3(S727) antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Phospho-STAT3(S727) antibody. Counter stained with hematoxylin.| Positive Control | A431, NIH/3T3, Hela, rat kidney tissue, rat liver tissue, human lung tissue, rat brain tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ . Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
