WB
Western blot analysis of alpha smooth muscle Actin on different lysates with Rabbit anti-alpha smooth muscle Actin antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate, Lane 2: A431 cell lysate, Lane 3: A549 cell lysate, Lane 4: NIH/3T3 cell lysate, Lane 5: C2C12 cell lysate, Lane 6: L6 cell lysate, Lane 7: Mouse colon tissue lysate, Lane 8: Rat colon tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.FC
Flow cytometric analysis of Hela cells with alpha smooth muscle Actin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | alpha smooth muscle Actin Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas –β and γ-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia. |
| Immunogen | Synthetic peptide within N-terminal human alpha smooth muscle Actin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | FCIHCWB |
| WB:1:1,000-1:5,000 IHC:1:50-1:200: FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | a actin antibody
AAT6 antibody
ACTA_HUMAN antibody
ACTA2 antibody
Actin alpha 2 smooth muscle aorta antibody
Actin aortic smooth muscle antibody
Actin aortic smooth muscle antibody ACTSA antibody ACTVS antibody Alpha 2 actin antibody Alpha actin 2 antibody Alpha cardiac actin antibody Alpha-actin-2 antibody Cell growth inhibiting gene 46 protein antibody Cell growth-inhibiting gene 46 protein antibody GIG46 antibody Growth inhibiting gene 46 antibody MYMY5 antibody α-SMA antibody |
|---|---|
| Molecular Weight(MW) | 42 kDa |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of alpha smooth muscle Actin on different lysates with Rabbit anti-alpha smooth muscle Actin antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate, Lane 2: A431 cell lysate, Lane 3: A549 cell lysate, Lane 4: NIH/3T3 cell lysate, Lane 5: C2C12 cell lysate, Lane 6: L6 cell lysate, Lane 7: Mouse colon tissue lysate, Lane 8: Rat colon tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.
FC
Flow cytometric analysis of Hela cells with alpha smooth muscle Actin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | A549, NIH/3T3, Hela, human tonsil tissue, human lung tissue, human liver tissue, mouse small intestine tissue, mouse skin tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 IHC:1:50-1:200: FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
