Application
Fig1: Western blot analysis of GSK3 beta on different lysates using anti-GSK3 beta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293 Lane 3: NIH/3T3 Lane 4: PC12Application
Fig2: ICC staining GSK3 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining GSK3 beta in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining GSK3 beta in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-GSK3 beta antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-GSK3 beta antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-GSK3 beta antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-GSK3 beta antibody. Counter stained with hematoxylin.Application
Fig9: Flow cytometric analysis of Hela cells with GSK3 beta antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | GSK3 beta [SY28-03] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Glycogen Synthase Kinase 3 Beta antibody Glycogen synthase kinase-3 beta antibody GSK 3 beta antibody GSK-3 beta antibody GSK3B antibody GSK3B_HUMAN antibody GSK3beta isoform antibody Serine/threonine-protein kinase GSK3B antibody |
| Product description | Glycogen synthase kinase 3, or GSK-3, is a serine/threonine, proline-directed kinase involved in a diverse array of signaling pathways, including glycogen synthesis and cellular adhesion, and has been implicated in Alzheimer’s disease. Two forms of GSK-3, designated GSK-3α and GSK-3β, have been identified and differ in their subcellular localization. Tau, a microtubule-binding protein which serves to stabilize microtubules in growing axons, is found to be hyper-phosphorylated in paired helical filaments (PHF), the major fibrous component of neurofibrillary lesions associated with Alzheimer’s disease. Hyperphosphorylation of Tau is thought to be the critical event leading to the assembly of PHF. Six Tau protein isoforms have been identified, all of which are phosphorylated by GSK-3. This presents the possibility that miscues in GSK-3 signaling contribute to the onset of Alzheimer’s disease. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | Glycogen Synthase Kinase 3 Beta antibody Glycogen synthase kinase-3 beta antibody GSK 3 beta antibody GSK-3 beta antibody GSK3B antibody GSK3B_HUMAN antibody GSK3beta isoform antibody Serine/threonine-protein kinase GSK3B antibody |
|---|---|
| Molecular Weight(MW) | 46 kDa |
| Cellular Localization | Cytoplasm, Nucleus, Cell membrane. |
Application
Fig1: Western blot analysis of GSK3 beta on different lysates using anti-GSK3 beta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293 Lane 3: NIH/3T3 Lane 4: PC12
Application
Fig2: ICC staining GSK3 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining GSK3 beta in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining GSK3 beta in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-GSK3 beta antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-GSK3 beta antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-GSK3 beta antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-GSK3 beta antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of Hela cells with GSK3 beta antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | PC-3M, SKOV-3, Hela, PC12, NIH/3T3, mouse prostate tissue, human breast tissue, human breast carcinoma tissue, mouse testis tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
